[ Identification et quantification de mycotoxines détectées à partir de différentes souches de champignons cultivés sur milieu de culture PDA en présence des œufs d’Achatina fulica (Bowdich) ]
Volume 17, Issue 4, September 2016, Pages 1263–1274
DEDI née KY Juliette1, OTCHOUMOU Atcho2, and ALLOU Kouassi3
1 Laboratoire de Biologie et Amélioration des Productions Végétales, Université Nangui Abrogoua, UFR-SN, Abidjan, Côte d'Ivoire
2 Laboratoire de Biologie et Cytologie Animales, Université Nangui Abrogoua, BP 801 Abidjan 02, Côte d'Ivoire
3 Centre National de Recherche Agronomique (CNRA), Station de recherche Marc Delorme, Laboratoire de Défense des cultures sur le programme cocotier, Abidjan, Côte d'Ivoire
Original language: French
Copyright © 2016 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The characterization of the fungal flora of the breeding litter Achatina fulica and different incubation substrates (coconut husks, cotton wool, sawdust, planting soil, virgin forest soil) of the eggs revealed the presence cosmopolitan fungi such as Aspergillus Niger, Fusarium oxysporum, Fusarium solani, Mucor sp., Penicillium decumbens, Penicillium sp. and Trichoderma sp. Note however that fusaria are only present on the breeding litter the giant African snail. The percentage of occurrence of fungi varies from one substrate to another. Aspergillus Niger remains the only fungus present on all substrates and Penicillium decumbens only on the planting soil. Similarly, the characterization of the potential of each of toxigenic strains performed through the identification technique by HPLC was able to detect and quantify ochratoxin A and aflatoxin G2, G1, B1 at most samples (eggs + fungus ) except that consisting of eggs + Fusarium solani or no mycotoxins were detected. Ochratoxin A detected in samples containing Aspergillus Niger, Oxysporum Fusarium, Mucor sp. and Penicillium sp. is present in very small quantities in the set. The aflatoxin B2 is absent in all samples.
Author Keywords: aflatoxins, ochratoxin A, fungi, incubation substrates, eggs.
Volume 17, Issue 4, September 2016, Pages 1263–1274
DEDI née KY Juliette1, OTCHOUMOU Atcho2, and ALLOU Kouassi3
1 Laboratoire de Biologie et Amélioration des Productions Végétales, Université Nangui Abrogoua, UFR-SN, Abidjan, Côte d'Ivoire
2 Laboratoire de Biologie et Cytologie Animales, Université Nangui Abrogoua, BP 801 Abidjan 02, Côte d'Ivoire
3 Centre National de Recherche Agronomique (CNRA), Station de recherche Marc Delorme, Laboratoire de Défense des cultures sur le programme cocotier, Abidjan, Côte d'Ivoire
Original language: French
Copyright © 2016 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The characterization of the fungal flora of the breeding litter Achatina fulica and different incubation substrates (coconut husks, cotton wool, sawdust, planting soil, virgin forest soil) of the eggs revealed the presence cosmopolitan fungi such as Aspergillus Niger, Fusarium oxysporum, Fusarium solani, Mucor sp., Penicillium decumbens, Penicillium sp. and Trichoderma sp. Note however that fusaria are only present on the breeding litter the giant African snail. The percentage of occurrence of fungi varies from one substrate to another. Aspergillus Niger remains the only fungus present on all substrates and Penicillium decumbens only on the planting soil. Similarly, the characterization of the potential of each of toxigenic strains performed through the identification technique by HPLC was able to detect and quantify ochratoxin A and aflatoxin G2, G1, B1 at most samples (eggs + fungus ) except that consisting of eggs + Fusarium solani or no mycotoxins were detected. Ochratoxin A detected in samples containing Aspergillus Niger, Oxysporum Fusarium, Mucor sp. and Penicillium sp. is present in very small quantities in the set. The aflatoxin B2 is absent in all samples.
Author Keywords: aflatoxins, ochratoxin A, fungi, incubation substrates, eggs.
Abstract: (french)
La caractérisation de la flore fongique de la litière d’élevage d’Achatina fulica et des différents substrats d’incubation (bourres de coco, coton hydrophile, sciure de bois, sol de plantation, sol de forêt vierge) des œufs a révélé la présence de champignons cosmopolites comme Aspergillus niger, Fusarium oxysporum, Fusarium solani , Mucor sp., Penicillium decumbens, Penicillium sp. et Trichoderma sp. Il faut cependant noter que les Fusarium ne sont présents que sur la litière d’élevage de l’escargot géant africain. Le pourcentage d’apparition des champignons est variable d’un substrat à un autre. Aspergillus niger reste le seul champignon présent sur tous les substrats et Penicillium decumbens uniquement sur le sol de plantation. De même, la caractérisation du potentiel toxinogène de chacune des souches, réalisée grâce à la technique d’identification par HPLC, a permis de détecter et quantifier l’ochratoxine A et les aflatoxines G2, G1, B1 au niveau de la majorité des échantillons (œufs + champignon) sauf celui constitué des œufs + Fusarium solani ou aucune des mycotoxines n’a été détectée. L’ochratoxine A, détectée au niveau des échantillons contenant Aspergillus niger, Fusarium Oxysporum, Mucor sp. et Penicillium sp. est présente en très faible quantité dans l’ensemble. L’aflatoxine B2 est absente au niveau de tous les échantillons.
Author Keywords: aflatoxines, ochratoxine A, mycètes, substrats d’incubation, œufs.
How to Cite this Article
DEDI née KY Juliette, OTCHOUMOU Atcho, and ALLOU Kouassi, “Identification and quantification of mycotoxins detected from different strains of fungi grown on PDA culture medium in the presence of eggs of Achatina fulica (Bowdich),” International Journal of Innovation and Applied Studies, vol. 17, no. 4, pp. 1263–1274, September 2016.
