[ Activités antiradicalaires et étude des composés volatils de trois plantes de la médecine traditionnelle du Bénin: Anchomanes difformis, Parkia biglobosa et Polyalthia longifolia ]
Volume 9, Issue 4, December 2014, Pages 1609–1619
Moukimou. L. AKIBOU OSSENI1, D. C. Pascal AGBANGNAN2, Annick BOSSOU3, H. Paul YEDOMONHAN4, Félicien AVLESSI5, and K. C. Dominique SOHOUNHLOUE6
1 Université d'Abomey-Calavi, Ecole Polytechnique d'Abomey-Calavi, Laboratoire d'Etude et de Recherche en Chimie Appliquée, 01 BP 2009 Cotonou, Benin
2 Université d'Abomey-Calavi, Ecole Polytechnique d'Abomey-Calavi, Laboratoire d'Etude et de Recherche en Chimie Appliquée, 01 BP 2009 Cotonou, Benin
3 Université d'Abomey-Calavi, Ecole Polytechnique d'Abomey-Calavi, Laboratoire d'Etude et de Recherche en Chimie Appliquée, 01 BP 2009 Cotonou, Benin
4 Université d'Abomey-Calavi, Département de Biologie Végétale, Faculté des Sciences et Technique, Herbier National, Benin
5 Université d'Abomey-Calavi, Ecole Polytechnique d'Abomey-Calavi, Laboratoire d'Etude et de Recherche en Chimie Appliquée, 01 BP 2009 Cotonou, Benin
6 Université d'Abomey-Calavi, Ecole Polytechnique d'Abomey-Calavi, Laboratoire d'Etude et de Recherche en Chimie Appliquée, 01 BP 2009 Cotonou, Benin
Original language: French
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The present study was devoted to the chemical analysis of three plants (Anchomanes difformis, Parkia biglobosa and Polyalthia longifolia) commonly used by traditional healers in Benin, for their curative properties in the treatment of certain diseases.
After characterization of large chemical groups present in the leaves of these plants, total polyphenols, condensed tannins, total flavonoids, anthocyanins and anthocyanidins were quantified by spectrophotometric methods. The scavenging activity was evaluated by the DPPH method and the volatile compounds in the powders of these plants were determined by SPME-GC/MS.
The results showed the presence of several secondary metabolites such as saponins, alkaloids, tannin, mucilages, anthraquinones, leucoanthocyanins, anthocyanins and triterpennoids in varying proportions in the three plants. The levels of condensed tannins, total flavonoids and anthocyanins are respectively higher in leaves of P. longifolia (22.5 mg/g, 15.69 mg / g and 0.94 mg/g) than in those of P. biglobosa (16.48 mg/g, 12.03 mg/g and 0.38 mg/g) and that of A. difformis (9.74 mg/g 4.59 mg/g and 0 mg/g).
Against by the total polyphenol content P.longifolia leaves (2.89 mg/g) is between those of A. difformis (2.19 mg/g) and P. biglobosa (4.5 mg/g). It is the same for the contents of anthocyanidins. Three plants studied, P. longifolia proved richest in polyphenols (condensed tannins, total flavonoids, anthocyanidins and anthocyanins) and A. difformis the less rich. This content of polyphenolic compounds would explain the test results of radical scavenging activities with an IC50 of 21.66 mg/mL for P. longifolia, 23.99 mg/mL for P. biglobosa and 26.5 mg/mL for A. difformis.
The solid phase micro extraction (SPME) coupled with GC/MS allowed the unambiguous identification of volatile components of each of the three plants.
Author Keywords: Polyphenols, Radical scavenging activities, SPME, Anchomanes difformis, Parkia biglobosa, Polyalthia longifolia.
Volume 9, Issue 4, December 2014, Pages 1609–1619
Moukimou. L. AKIBOU OSSENI1, D. C. Pascal AGBANGNAN2, Annick BOSSOU3, H. Paul YEDOMONHAN4, Félicien AVLESSI5, and K. C. Dominique SOHOUNHLOUE6
1 Université d'Abomey-Calavi, Ecole Polytechnique d'Abomey-Calavi, Laboratoire d'Etude et de Recherche en Chimie Appliquée, 01 BP 2009 Cotonou, Benin
2 Université d'Abomey-Calavi, Ecole Polytechnique d'Abomey-Calavi, Laboratoire d'Etude et de Recherche en Chimie Appliquée, 01 BP 2009 Cotonou, Benin
3 Université d'Abomey-Calavi, Ecole Polytechnique d'Abomey-Calavi, Laboratoire d'Etude et de Recherche en Chimie Appliquée, 01 BP 2009 Cotonou, Benin
4 Université d'Abomey-Calavi, Département de Biologie Végétale, Faculté des Sciences et Technique, Herbier National, Benin
5 Université d'Abomey-Calavi, Ecole Polytechnique d'Abomey-Calavi, Laboratoire d'Etude et de Recherche en Chimie Appliquée, 01 BP 2009 Cotonou, Benin
6 Université d'Abomey-Calavi, Ecole Polytechnique d'Abomey-Calavi, Laboratoire d'Etude et de Recherche en Chimie Appliquée, 01 BP 2009 Cotonou, Benin
Original language: French
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The present study was devoted to the chemical analysis of three plants (Anchomanes difformis, Parkia biglobosa and Polyalthia longifolia) commonly used by traditional healers in Benin, for their curative properties in the treatment of certain diseases.
After characterization of large chemical groups present in the leaves of these plants, total polyphenols, condensed tannins, total flavonoids, anthocyanins and anthocyanidins were quantified by spectrophotometric methods. The scavenging activity was evaluated by the DPPH method and the volatile compounds in the powders of these plants were determined by SPME-GC/MS.
The results showed the presence of several secondary metabolites such as saponins, alkaloids, tannin, mucilages, anthraquinones, leucoanthocyanins, anthocyanins and triterpennoids in varying proportions in the three plants. The levels of condensed tannins, total flavonoids and anthocyanins are respectively higher in leaves of P. longifolia (22.5 mg/g, 15.69 mg / g and 0.94 mg/g) than in those of P. biglobosa (16.48 mg/g, 12.03 mg/g and 0.38 mg/g) and that of A. difformis (9.74 mg/g 4.59 mg/g and 0 mg/g).
Against by the total polyphenol content P.longifolia leaves (2.89 mg/g) is between those of A. difformis (2.19 mg/g) and P. biglobosa (4.5 mg/g). It is the same for the contents of anthocyanidins. Three plants studied, P. longifolia proved richest in polyphenols (condensed tannins, total flavonoids, anthocyanidins and anthocyanins) and A. difformis the less rich. This content of polyphenolic compounds would explain the test results of radical scavenging activities with an IC50 of 21.66 mg/mL for P. longifolia, 23.99 mg/mL for P. biglobosa and 26.5 mg/mL for A. difformis.
The solid phase micro extraction (SPME) coupled with GC/MS allowed the unambiguous identification of volatile components of each of the three plants.
Author Keywords: Polyphenols, Radical scavenging activities, SPME, Anchomanes difformis, Parkia biglobosa, Polyalthia longifolia.
How to Cite this Article
Moukimou. L. AKIBOU OSSENI, D. C. Pascal AGBANGNAN, Annick BOSSOU, H. Paul YEDOMONHAN, Félicien AVLESSI, and K. C. Dominique SOHOUNHLOUE, “Radical scavenging activities and study of volatile compounds of three plants used in traditional medicine in Benin: Anchomanes difformis, Parkia biglobosa and Polyalthia longifolia,” International Journal of Innovation and Applied Studies, vol. 9, no. 4, pp. 1609–1619, December 2014.
