[ تأثير بعض المستخلصات المائية (نبات خناق الدجاج واوراق نبات اليوكالبتوس واوراق نبات الآس) على طفيلي الأميبا الحالة للنسيج المنمى في الوسط الزرعي خلاصة كبد الابقار ]
Volume 10, Issue 4, March 2015, Pages 1076–1085
Sahar Abass Hussien1, Hadi Mezeal Khudhair2, and Ahmed Khudhair AL-Hamairy3
1 Biology, Babylon/Science College for Women, Al-Hilla, Babylon Province, Iraq
2 Biology, Babylon/Science College for Women, Al-Hilla, Babylon Province, Iraq
3 Biology, Babylon/Science College for Women, Al-Hilla, Babylon Province, Iraq
Original language: Arabic
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The present study has been conducted in Babylon/Science College for Women to detect the efficiency of three plant extracts (Euphorbia helioscobia, Eucalyptus globules and Myrtus comminus on the Entamoeba histolytica Parasites that grown in beef liver infusion medium at 37 centigreat temperature. Concentration of cooled and boiled plant extracts its(2.5 ,5 , 10) % of the three plants as well as control group(distelled water). The results revealed that the boiled plant extracts of three plants were the efficient from cooled plant extracts in killing the trophozoites of the Entamoeba histolytica . The E. helioscobia boiled plant extracts was the efficient to killing the trophozoites in the second day from the begining of the experiment with 10 mgml. And the next its boiled plant extracts of Eucalyptus globules and Myrtus comminus in the third day from the begining of the experiment with 5 mgml for each one .and all the three plant extracts inhibited the viability of E. histolytica that grown in beef liver infusion medium comparison with control group.as well as the metronidazole drug in one microliterml. finished the viability of trophozoites at the third day, and showed from the present study successfully grown E. histolytica in beef liver infusion medium.
Author Keywords: Amebiasis, Metronidazole, grown Entamoeba histolytica in media culture, plant extracts.
Volume 10, Issue 4, March 2015, Pages 1076–1085
Sahar Abass Hussien1, Hadi Mezeal Khudhair2, and Ahmed Khudhair AL-Hamairy3
1 Biology, Babylon/Science College for Women, Al-Hilla, Babylon Province, Iraq
2 Biology, Babylon/Science College for Women, Al-Hilla, Babylon Province, Iraq
3 Biology, Babylon/Science College for Women, Al-Hilla, Babylon Province, Iraq
Original language: Arabic
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The present study has been conducted in Babylon/Science College for Women to detect the efficiency of three plant extracts (Euphorbia helioscobia, Eucalyptus globules and Myrtus comminus on the Entamoeba histolytica Parasites that grown in beef liver infusion medium at 37 centigreat temperature. Concentration of cooled and boiled plant extracts its(2.5 ,5 , 10) % of the three plants as well as control group(distelled water). The results revealed that the boiled plant extracts of three plants were the efficient from cooled plant extracts in killing the trophozoites of the Entamoeba histolytica . The E. helioscobia boiled plant extracts was the efficient to killing the trophozoites in the second day from the begining of the experiment with 10 mgml. And the next its boiled plant extracts of Eucalyptus globules and Myrtus comminus in the third day from the begining of the experiment with 5 mgml for each one .and all the three plant extracts inhibited the viability of E. histolytica that grown in beef liver infusion medium comparison with control group.as well as the metronidazole drug in one microliterml. finished the viability of trophozoites at the third day, and showed from the present study successfully grown E. histolytica in beef liver infusion medium.
Author Keywords: Amebiasis, Metronidazole, grown Entamoeba histolytica in media culture, plant extracts.
Abstract: (arabic)
أُجريت الدراسة الحالية في مختبرات كلية العلوم للبنات / جامعة بابل للكشف عن تأثير المستخلصات المائية لنبات خناق الدجاج واوراق نباتات اليوكالبتوس والآس في النسبة المئوية لحيوية الأطوار الخضرية لطفيلي أميبا الحالة للنسيج والمنمى في الوسط الزرعي عند درجة حرارة 37 مo, تم الاستخلاص بالماء البارد والمغلي وحُضرت التراكيز (10, 5 , 2.5) % لكل من النباتات الثلاثة كلا على حده بالإضافة الى معامل السيطرة (الماء المقطر) .أظهرت النتائج ان تأثير المستخلص المائي المغلي لنباتات الثلاثة كان الأكثر تأثيراً في قتل الأطوار الخضرية لطفيلي الأميبا الحالة للنسيج من المستخلص الماء البارد , وبالنسبة للمستخلصات المائية المغلية ,فقد كان مستخلص نبات خناق الدجاج الأكفأ في قتل الأطوار الخضرية في اليوم الثاني من بدء التجربة بتركيز 10 ملغم / مليلتر وجاء بعده مستخلص اليوكالبتوس للماء المغلي والآس للماء المغلي في اليوم الثالث بتركيز 5 ملغم/ مليلتر لكل منهما, وان جميع تراكيز المستخلصات المائية لنباتات قيد الدراسة ثبطت النسبة المئوية لحيوية الأطوار الخضرية لطفيلي الأميبا الحالة للنسيج بالمقارنة مع معامل السيطرة . وعقار الميترونيدازول كان له تأثير أيضا في القضاء على الطفيلي في اليوم الثالث بتركيز 1 مكغم / مليلتر, وتبين من الدراسة الحالية نجاح الوسط الزرعي في تنمية الأميبا الحالة للنسيج لبضعة أيام فقط وانتهت حيويتها في اليوم السادس من التجرية .
Author Keywords: داء الاميبات, عقار الميترونيدازول, نمو الاميبا الحالة للنسيج في الوسط الزرعي, مستخلصات نباتية.
How to Cite this Article
Sahar Abass Hussien, Hadi Mezeal Khudhair, and Ahmed Khudhair AL-Hamairy, “Effect of Some Water Plant Extracts(Euphorbia helioscobia and leaves of Eucalyptus globules and Myrtus comminus on The Entamoeba histolytica Parasites That Grown in Beef Liver Infusion Medium,” International Journal of Innovation and Applied Studies, vol. 10, no. 4, pp. 1076–1085, March 2015.
