Volume 18, Issue 3, November 2016, Pages 743–748
Sara T. Elazab1, Mohamed G. Gabr2, Magdy S. Amer3, Sameh M. El-nabtity4, and Walter H. Hsu5
1 Department of Pharmacology, Faculty of Veterinary Medicine, Mansoura University, El-Mansoura, Egypt
2 Department of Pharmacology, Faculty of Veterinary Medicine, Mansoura University, El-Mansoura, Egypt
3 Department of Pharmacology, Faculty of Veterinary Medicine, Mansoura University, El-Mansoura, Egypt
4 Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Egypt
5 Department of Biomedical Sciences, College of veterinary Medicine, Iowa State University, Ames, Iowa, USA
Original language: English
Copyright © 2016 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The current study was carried out in 24 New Zealand white rabbits (2.0-2.5 kg) to evaluate cefquinome residues in their tissues (kidney, liver, and muscle) following intramuscular (IM) administration of 2 mg/kg of body weight, once daily for 3 consecutive days. The solid phase extraction and high performance liquid chromatography were used to determine cefquinome concentrations in tissue samples. We found that cefquinome was highly concentrated in the kidney followed by the liver, while traces of it were detected in muscles. Cefquinome was not detected in kidney on the 24th day post-administration, while it disappeared from liver and muscle tissues on 7th and 3rdday post-administration, respectively. The withdrawal periods were established based on European UnionMaximum Residue Levels (EU MRL) using the statistical method (95% tolerance limit and 95% confidence) stated in the guidance and the withdrawal time calculation program WT1.4 which was developed by Germany and adopted by the Committee for Veterinary Medicinal Products (CVMP) of EU. The suggested preslaughter periods are: 10 days for kidney, 2 days for liver and 1 day for muscle.
Author Keywords: Cefquinome, Residue, Withdrawal time, HPLC, EU MRL.
Sara T. Elazab1, Mohamed G. Gabr2, Magdy S. Amer3, Sameh M. El-nabtity4, and Walter H. Hsu5
1 Department of Pharmacology, Faculty of Veterinary Medicine, Mansoura University, El-Mansoura, Egypt
2 Department of Pharmacology, Faculty of Veterinary Medicine, Mansoura University, El-Mansoura, Egypt
3 Department of Pharmacology, Faculty of Veterinary Medicine, Mansoura University, El-Mansoura, Egypt
4 Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Egypt
5 Department of Biomedical Sciences, College of veterinary Medicine, Iowa State University, Ames, Iowa, USA
Original language: English
Copyright © 2016 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The current study was carried out in 24 New Zealand white rabbits (2.0-2.5 kg) to evaluate cefquinome residues in their tissues (kidney, liver, and muscle) following intramuscular (IM) administration of 2 mg/kg of body weight, once daily for 3 consecutive days. The solid phase extraction and high performance liquid chromatography were used to determine cefquinome concentrations in tissue samples. We found that cefquinome was highly concentrated in the kidney followed by the liver, while traces of it were detected in muscles. Cefquinome was not detected in kidney on the 24th day post-administration, while it disappeared from liver and muscle tissues on 7th and 3rdday post-administration, respectively. The withdrawal periods were established based on European UnionMaximum Residue Levels (EU MRL) using the statistical method (95% tolerance limit and 95% confidence) stated in the guidance and the withdrawal time calculation program WT1.4 which was developed by Germany and adopted by the Committee for Veterinary Medicinal Products (CVMP) of EU. The suggested preslaughter periods are: 10 days for kidney, 2 days for liver and 1 day for muscle.
Author Keywords: Cefquinome, Residue, Withdrawal time, HPLC, EU MRL.
How to Cite this Article
Sara T. Elazab, Mohamed G. Gabr, Magdy S. Amer, Sameh M. El-nabtity, and Walter H. Hsu, “Depletion of cefquinome from rabbit tissues,” International Journal of Innovation and Applied Studies, vol. 18, no. 3, pp. 743–748, November 2016.
