Volume 22, Issue 1, December 2017, Pages 78–85
VEMO Bertin Narcisse1, KENFACK Augustave2, NJINGOU NDOUOYA Badou Zaki3, NGOULA Ferdinand4, NANTIA AKONO Edouard5, TSAMBOU MEGNIMEZA Astride Martine6, GUIEKEP NOUNAMO Arthénice Jemima7, NELO Chancel Patrick8, NGALEU NJIEUDEU Claude Cedric9, YIDJEU NANA Ferry10, and Teguia Alexis11
1 University of Dschang, Faculty of Agronomy and Agricultural Sciences, Department of Animal Sciences, PO Box 188, Cameroon
2 University of Dschang, Faculty of Agronomy and Agricultural Sciences, Department of Animal Sciences, PO Box 188, Cameroon
3 University of Dschang, Faculty of Agronomy and Agricultural Sciences, Department of Animal Sciences, PO Box 188, Cameroon
4 University of Dschang, Faculty of Agronomy and Agricultural Sciences, Department of Animal Sciences, PO Box 188, Cameroon
5 University of Bamenda, Faculty of Sciences, Department of Biochemistry, PO Box 39, Bambili, Cameroon
6 University of Dschang, Faculty of Agronomy and Agricultural Sciences, Department of Animal Sciences, PO Box 188, Cameroon
7 University of Dschang, Faculty of Agronomy and Agricultural Sciences, Department of Animal Sciences, PO Box 188, Cameroon
8 University of Dschang, Faculty of Agronomy and Agricultural Sciences, Department of Animal Sciences, PO Box 188, Cameroon
9 University of Dschang, Faculty of Agronomy and Agricultural Sciences, Department of Animal Sciences, PO Box 188, Cameroon
10 University of Dschang, Faculty of Agronomy and Agricultural Sciences, Department of Animal Sciences, PO Box 188, Cameroon
11 Département des Production Animales, Faculté d'Agronomie et des Sciences Agricoles, Université de Dschang, BP: 70 Dschang, Cameroon
Original language: English
Copyright © 2017 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The present study aimed to evaluate the protective effects of aqueous extract of Bersama engleriana leaves against cypermethrin-induced oxidative stress and reproductive toxicity. Thus, fifty male guinea pigs were divided into 5 groups (G1, G2, G3, G4 and G5) of 10 animals each. During 90 days, animals of G1 were orally given 1 ml/kg of body weight (bw)/day of distilled water, while the other groups received 137.50 mg/kg bw of cypermethrin. In addition, G3, G4 and G5 received respectively 50, 100 and 200 mg/kg bw of aqueous extract of Bersama engleriana leaves. At the end of the treatment period, the testicular concentration of malondialdehyde and the activities of superoxide dismutase and catalase decreased significantly (P<0.05) in guinea pigs exposed to cypermethrin and aqueous extract of B. engleriana leaves compared to those exposed to cypermethrin only (G2), while the reverse was observed with the activity of peroxidases. The time of reaction of male guinea pig in the presence of females and the percentage of abnormal spermatozoa decreased significantly (P<0.05) in animals treated with the insecticide and the aqueous extract of B. engleriana leaves compared to G2 animals. The weight of testes, serum level of testosterone, sperm count, mobility and percentage of spermatozoa with entire plasma membrane increased significantly (P<0.05) in guinea pigs given extract compared to those submitted to cypermethrin only. Thus, the aqueous extract of Bersama engleriana leaves protected male guinea pigs against cypermethrin toxicity.
Author Keywords: Bersama engleriana, cypermethin, oxidative stress, spermatozoa, toxicity.
VEMO Bertin Narcisse1, KENFACK Augustave2, NJINGOU NDOUOYA Badou Zaki3, NGOULA Ferdinand4, NANTIA AKONO Edouard5, TSAMBOU MEGNIMEZA Astride Martine6, GUIEKEP NOUNAMO Arthénice Jemima7, NELO Chancel Patrick8, NGALEU NJIEUDEU Claude Cedric9, YIDJEU NANA Ferry10, and Teguia Alexis11
1 University of Dschang, Faculty of Agronomy and Agricultural Sciences, Department of Animal Sciences, PO Box 188, Cameroon
2 University of Dschang, Faculty of Agronomy and Agricultural Sciences, Department of Animal Sciences, PO Box 188, Cameroon
3 University of Dschang, Faculty of Agronomy and Agricultural Sciences, Department of Animal Sciences, PO Box 188, Cameroon
4 University of Dschang, Faculty of Agronomy and Agricultural Sciences, Department of Animal Sciences, PO Box 188, Cameroon
5 University of Bamenda, Faculty of Sciences, Department of Biochemistry, PO Box 39, Bambili, Cameroon
6 University of Dschang, Faculty of Agronomy and Agricultural Sciences, Department of Animal Sciences, PO Box 188, Cameroon
7 University of Dschang, Faculty of Agronomy and Agricultural Sciences, Department of Animal Sciences, PO Box 188, Cameroon
8 University of Dschang, Faculty of Agronomy and Agricultural Sciences, Department of Animal Sciences, PO Box 188, Cameroon
9 University of Dschang, Faculty of Agronomy and Agricultural Sciences, Department of Animal Sciences, PO Box 188, Cameroon
10 University of Dschang, Faculty of Agronomy and Agricultural Sciences, Department of Animal Sciences, PO Box 188, Cameroon
11 Département des Production Animales, Faculté d'Agronomie et des Sciences Agricoles, Université de Dschang, BP: 70 Dschang, Cameroon
Original language: English
Copyright © 2017 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The present study aimed to evaluate the protective effects of aqueous extract of Bersama engleriana leaves against cypermethrin-induced oxidative stress and reproductive toxicity. Thus, fifty male guinea pigs were divided into 5 groups (G1, G2, G3, G4 and G5) of 10 animals each. During 90 days, animals of G1 were orally given 1 ml/kg of body weight (bw)/day of distilled water, while the other groups received 137.50 mg/kg bw of cypermethrin. In addition, G3, G4 and G5 received respectively 50, 100 and 200 mg/kg bw of aqueous extract of Bersama engleriana leaves. At the end of the treatment period, the testicular concentration of malondialdehyde and the activities of superoxide dismutase and catalase decreased significantly (P<0.05) in guinea pigs exposed to cypermethrin and aqueous extract of B. engleriana leaves compared to those exposed to cypermethrin only (G2), while the reverse was observed with the activity of peroxidases. The time of reaction of male guinea pig in the presence of females and the percentage of abnormal spermatozoa decreased significantly (P<0.05) in animals treated with the insecticide and the aqueous extract of B. engleriana leaves compared to G2 animals. The weight of testes, serum level of testosterone, sperm count, mobility and percentage of spermatozoa with entire plasma membrane increased significantly (P<0.05) in guinea pigs given extract compared to those submitted to cypermethrin only. Thus, the aqueous extract of Bersama engleriana leaves protected male guinea pigs against cypermethrin toxicity.
Author Keywords: Bersama engleriana, cypermethin, oxidative stress, spermatozoa, toxicity.
How to Cite this Article
VEMO Bertin Narcisse, KENFACK Augustave, NJINGOU NDOUOYA Badou Zaki, NGOULA Ferdinand, NANTIA AKONO Edouard, TSAMBOU MEGNIMEZA Astride Martine, GUIEKEP NOUNAMO Arthénice Jemima, NELO Chancel Patrick, NGALEU NJIEUDEU Claude Cedric, YIDJEU NANA Ferry, and Teguia Alexis, “Protective effects of the aqueous extract of Bersama engleriana leaves against cypermethrin-induced oxidative stress and reproductive toxicity in male guinea pig (Cavia porcellus),” International Journal of Innovation and Applied Studies, vol. 22, no. 1, pp. 78–85, December 2017.
