[ Analyse de la diversité des rhizobiums nodulant trois morphotypes Nigériens de Voandzou (Vigna Subterranea (L.) Verdc.) par la technique de PCR-RFLP ]
Volume 24, Issue 2, September 2018, Pages 742–754
Harouna Issa Amadou1, Hadjara AMADOU HASSANE2, Alhassane Agali3, Mansour ABDOU MAMAN4, and Zoubeirou ALZOUMA MAYAKI5
1 Laboratoire de Gestion et Valorisation de la Biodiversité au Sahel (GeVaBioS), Faculté des Sciences et Techniques, BP 10662 Ny, Université Abdou Moumouni, Niger
2 Département de Biologie, Faculté des Sciences et Techniques, Université Abdou Moumouni, BP 10662 Niamey, Niger
3 Département Information et Recherche, Centre AGRHYMET CCR/AOS, BP 11011 Niamey, Niger
4 Département de Production Durable des Cultures, Université de Tillabéri, Niger
5 Université Abdou Moumouni de Niamey, Faculté des Sciences et Techniques, BP. 10662 Niamey, Niger
Original language: French
Copyright © 2018 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Bambara groundnut [Vigna Subterranea (L.) Verdc.], plays an important role in increasing the bioavailability of phosphorus even in ferrolsoils through its ability to fix atmospheric nitrogen and gives an average yields ranging from 350 to 800 kg / ha in areas where soil is poor and rainfall is low. This ability to bind atmospheric nitrogen by legumes such as Bambara groundnut [Vigna subterranea (L.)) is due to the presence of root nodules harboring colonies of Rhizobium, symbiotic bacteria. Indeed, it has been showed some diversity in the isolated nodules from the same legume and proved that, apart from the genetic difference, some strains are more efficient, more competitive than others. The objective of this study is to evaluate the diversity of the nodulating rhizobia of Bambara groundnut morphotypes of Niger. For that, the PCR / RFLP technique was used to identify the polymorphism between rhizobium strains isolated from the root nodules of three (3) morphotypes (Ne-01, Ne-09 and Ne-10) cultivated at two sites (Tara and Kollo) of two different agro-ecological areas. The analysis revealed a high diversity within populations of rhizobia nodulating of Vigna Subterranea L. A total of twenty five (25) types of IGS profiles were identified from the 68 samples analyzed with 4 dominant types (II, XI, XIV and XVI). In general, the same types of IGS for the same morphotype at both sites were observed. It also appeared that the growing area has an impact on rhizobia diversity. Thus, it has been shown that the genetic diversity of rhizobia populations is much higher in Tara (20/25 types identified) compared to Kollo (12/25 types). The distribution of rhizobia populations capable of nodulating the Vigna Subterranea L. was depended not only at the morphotype but also at the growing area.
Author Keywords: Bambara groundnut, diversity, rhizobium, PCR / RFLP, Niger.
Volume 24, Issue 2, September 2018, Pages 742–754
Harouna Issa Amadou1, Hadjara AMADOU HASSANE2, Alhassane Agali3, Mansour ABDOU MAMAN4, and Zoubeirou ALZOUMA MAYAKI5
1 Laboratoire de Gestion et Valorisation de la Biodiversité au Sahel (GeVaBioS), Faculté des Sciences et Techniques, BP 10662 Ny, Université Abdou Moumouni, Niger
2 Département de Biologie, Faculté des Sciences et Techniques, Université Abdou Moumouni, BP 10662 Niamey, Niger
3 Département Information et Recherche, Centre AGRHYMET CCR/AOS, BP 11011 Niamey, Niger
4 Département de Production Durable des Cultures, Université de Tillabéri, Niger
5 Université Abdou Moumouni de Niamey, Faculté des Sciences et Techniques, BP. 10662 Niamey, Niger
Original language: French
Copyright © 2018 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Bambara groundnut [Vigna Subterranea (L.) Verdc.], plays an important role in increasing the bioavailability of phosphorus even in ferrolsoils through its ability to fix atmospheric nitrogen and gives an average yields ranging from 350 to 800 kg / ha in areas where soil is poor and rainfall is low. This ability to bind atmospheric nitrogen by legumes such as Bambara groundnut [Vigna subterranea (L.)) is due to the presence of root nodules harboring colonies of Rhizobium, symbiotic bacteria. Indeed, it has been showed some diversity in the isolated nodules from the same legume and proved that, apart from the genetic difference, some strains are more efficient, more competitive than others. The objective of this study is to evaluate the diversity of the nodulating rhizobia of Bambara groundnut morphotypes of Niger. For that, the PCR / RFLP technique was used to identify the polymorphism between rhizobium strains isolated from the root nodules of three (3) morphotypes (Ne-01, Ne-09 and Ne-10) cultivated at two sites (Tara and Kollo) of two different agro-ecological areas. The analysis revealed a high diversity within populations of rhizobia nodulating of Vigna Subterranea L. A total of twenty five (25) types of IGS profiles were identified from the 68 samples analyzed with 4 dominant types (II, XI, XIV and XVI). In general, the same types of IGS for the same morphotype at both sites were observed. It also appeared that the growing area has an impact on rhizobia diversity. Thus, it has been shown that the genetic diversity of rhizobia populations is much higher in Tara (20/25 types identified) compared to Kollo (12/25 types). The distribution of rhizobia populations capable of nodulating the Vigna Subterranea L. was depended not only at the morphotype but also at the growing area.
Author Keywords: Bambara groundnut, diversity, rhizobium, PCR / RFLP, Niger.
Abstract: (french)
Le voandzou joue un rôle important sur l’augmentation de la biodisponibilité du phosphore même dans les ferralsols par le biais de sa capacité de fixation de l’azote atmosphérique et donne des rendements moyens allant de 350 à 800 kg/ha dans les régions où le sol est pauvre et la pluviométrie faible. Cette faculté de fixation de l’azote atmosphérique par les légumineuses comme le Voandzou [Vigna subterranea (L.), est due à la présence de nodosités racinaires hébergeant des colonies de Rhizobium, bactéries symbiotiques. En effet, il a été démontré une diversité au sein de nodules isolés provenant de la même légumineuse et prouvé que, hormis la différence génétique, certaines souches sont plus efficaces, plus compétitives que d'autres. L’objectif de cette étude est d’évaluer la diversité des Rhizobiums nodulants des morphotypes de voandzou du Niger. Pour ce faire la technique PCR/RFLP a été utilisée afin d’identifier le polymorphisme existant entre souches de rhizobium isolés à partir des nodules racinaires de trois (3) morphotypes (Ne-01, Ne-09 et Ne-10) de voandzou cultivés sur deux sites (Tara et Kollo) de deux zones agro-écologiques différentes. L’analyse a révélé une diversité élevée au sein des populations de rhizobia nodulant de voandzou. A total vingt-cinq (25) types de profils IGS ont été identifiés sur les 68 échantillons analysés avec 4 types (II, XI, XIV et XVI) dominants. De façon générale, les mêmes types d’IGS pour le même morphotype sur les deux sites ont été observés. Il ressort également que la zone de culture a un impact sur la diversité des rhizobia. Ainsi, elle a montré que la diversité génétique des populations de rhizobia est beaucoup plus élevée à Tara (20/25 types identifiés) comparativement à Kollo (12/25 types). La distribution des populations de rhizobia capables de noduler le voandzou dépende non seulement du morphotype également du site de culture.
Author Keywords: Voandzou, diversité, rhizobium, PCR/RFLP, Niger.
How to Cite this Article
Harouna Issa Amadou, Hadjara AMADOU HASSANE, Alhassane Agali, Mansour ABDOU MAMAN, and Zoubeirou ALZOUMA MAYAKI, “Diversity analysis of rhizobia nodulating three Nigerien morphotypes of Bambara groundnut (Vigna Subterranea (L.) Verdc.) By the PCR-RFLP technique,” International Journal of Innovation and Applied Studies, vol. 24, no. 2, pp. 742–754, September 2018.
