[ Analyse des fractions C3-C4 du complément: Corrélation entre la méthode électrophorétique et le dosage turbidimétrique ]
Volume 39, Issue 3, May 2023, Pages 1244–1247
H. Kouame1, Asmaa Drissi Bourhanbour2, Jalila Elbakkouri3, A. Morjan4, and N. Kamal5
1 Laboratoire de biochimie, CHU Ibn Rochd de Casablanca, Morocco
2 Laboratoire d’immunologie-sérologie, CHU Ibn Rochd Casablanca, Morocco
3 Clinical Immunology, Autoimmunity and Inflammation Laboratory (LICIA), Faculty of Medicine and Pharmacy, Hassan II University, Casablanca, Morocco
4 Laboratoire de biochimie, Hôpital Ibn Rochd, CHU Ibn Rochd, Casablanca, Morocco
5 Laboratoire de biochimie, Hôpital Ibn Rochd, CHU Ibn Rochd, Casablanca, Morocco
Original language: French
Copyright © 2023 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Complement is part of the host’s natural defense mechanisms against pathogens. Its exploration is based in first intention on a quantitative evaluation of the C3 and C4 fractions by automated and standardized immunoassay techniques. Serum protein electrophoresis (SPEP) separates proteins into 6 fractions. The beta-2 globulin fraction contains complement C3-C4, the amplitude of which allows their quantification. In this context, we carried out a comparative study between the two assay techniques. We included all patients who had simultaneously received a weight determination of the C3 and C4 fractions by turbidimetry on a SPA Plus® automaton and an SPEP on a Capillarys Sebia® automaton over a period of one year. Our study demonstrated a positive correlation between these two methods with Pearson r=0.801, P-value<0.001. Studies have reported that SPEP can be used for the detection of hypocomplementemia by a decrease in the fraction of beta-2 globulins. In capillary electrophoresis (Capillarys Sebia®), beta-2 globulins contain almost exclusively complement. To date, our study is the first to seek the correlation between two electrophoretic and turbidimetric methods for the quantification of complement.
Author Keywords: Complement system, capillary electrophoresis, turbidimetry.
Volume 39, Issue 3, May 2023, Pages 1244–1247
H. Kouame1, Asmaa Drissi Bourhanbour2, Jalila Elbakkouri3, A. Morjan4, and N. Kamal5
1 Laboratoire de biochimie, CHU Ibn Rochd de Casablanca, Morocco
2 Laboratoire d’immunologie-sérologie, CHU Ibn Rochd Casablanca, Morocco
3 Clinical Immunology, Autoimmunity and Inflammation Laboratory (LICIA), Faculty of Medicine and Pharmacy, Hassan II University, Casablanca, Morocco
4 Laboratoire de biochimie, Hôpital Ibn Rochd, CHU Ibn Rochd, Casablanca, Morocco
5 Laboratoire de biochimie, Hôpital Ibn Rochd, CHU Ibn Rochd, Casablanca, Morocco
Original language: French
Copyright © 2023 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Complement is part of the host’s natural defense mechanisms against pathogens. Its exploration is based in first intention on a quantitative evaluation of the C3 and C4 fractions by automated and standardized immunoassay techniques. Serum protein electrophoresis (SPEP) separates proteins into 6 fractions. The beta-2 globulin fraction contains complement C3-C4, the amplitude of which allows their quantification. In this context, we carried out a comparative study between the two assay techniques. We included all patients who had simultaneously received a weight determination of the C3 and C4 fractions by turbidimetry on a SPA Plus® automaton and an SPEP on a Capillarys Sebia® automaton over a period of one year. Our study demonstrated a positive correlation between these two methods with Pearson r=0.801, P-value<0.001. Studies have reported that SPEP can be used for the detection of hypocomplementemia by a decrease in the fraction of beta-2 globulins. In capillary electrophoresis (Capillarys Sebia®), beta-2 globulins contain almost exclusively complement. To date, our study is the first to seek the correlation between two electrophoretic and turbidimetric methods for the quantification of complement.
Author Keywords: Complement system, capillary electrophoresis, turbidimetry.
Abstract: (french)
Le complément fait partie des mécanismes naturels de défense de l’hôte contre les agents pathogènes. Son exploration se base en première intention sur une évaluation quantitative des fractions C3 et C4 par des techniques d’immunodosages automatisées et standardisées. L’électrophorèse des protéines sériques (EPS) sépare les protéines en 6 fractions. La fraction beta-2 globulines renferme du complément C3-C4, dont l’amplitude permet leur quantification. Dans ce contexte, nous avons réalisé une étude comparative entre les deux techniques de dosage. Nous avons inclus tous les patients ayant bénéficiés simultanément d’un dosage pondéral des fractions C3 et C4 par turbidimétrie sur automate SPA Plus® et d’une EPS sur automate Capillarys Sebia® sur une durée d’un an. Notre étude a démontré une corrélation positive entre ces deux méthodes avec Pearson r=0,801, P-value<0,001. Des études ont rapporté que l’EPS peut être utilisée pour la détection d’une hypocomplémentémie par une diminution de la fraction de beta-2 globulines. Dans l’électrophorèse capillaire par (Capillarys Sebia®), beta-2 globulines comporte presque exclusivement le complément. A ce jour, notre étude est la première à rechercher la corrélation entre deux méthodes électrophorétique et turbidimétrique pour la quantification du complément.
Author Keywords: Système complément, électrophorèse capillaire, turbidimétrie.
How to Cite this Article
H. Kouame, Asmaa Drissi Bourhanbour, Jalila Elbakkouri, A. Morjan, and N. Kamal, “Analysis of complement C3-C4 fractions: Correlation between the electrophoretic method and the turbidimetric assay,” International Journal of Innovation and Applied Studies, vol. 39, no. 3, pp. 1244–1247, May 2023.
