[ Vérification de la méthode de dosage sérique et urinaire du Glucose par méthode enzymatique à l’hexokinase sur l’automate Alinity au laboratoire de biochimie-CHU Ibn Rochd de Casablanca, Maroc ]
Volume 38, Issue 1, November 2022, Pages 86–92
D. Benzekri1, A. Boujmad2, Asmaa Morjan3, and N. Kamal4
1 Laboratoire de biochimie, CHU Ibn Rochd de Casablanca, Morocco
2 Laboratoire de biochimie, CHU Ibn Rochd de Casablanca, Morocco
3 Laboratoire de biochimie, CHU Ibn Rochd, Casablanca, Morocco
4 Laboratoire de biochimie, Hôpital Ibn Rochd, CHU Ibn Rochd, Casablanca, Morocco
Original language: French
Copyright © 2022 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The verification/validation of a method is a necessary prerequisite before any activity in a medical biology laboratory. Also, it is important to remember the control of the risks associated with any dosing method as well as the impact of pre-analytical conditions on the post-analytical step. The objective of this work is to evaluate the analytical performance of serum and urinary glucose assay using the hexokinase enzymatic method of the manufacturer Abbott on the Alinity automaton of the same manufacturer. The verified parameters are reproducibility and repeatability, through the results of internal quality controls retrieved by creating procedures in the section of validation/verification of methods in our software: Big. The results of the performance evaluation in terms of reproducibility and repeatability demonstrate a perfect compliance of this method of serum and urine glucose determination by the Abbott kit on the Alinity PLC of the same manufacturer.
Author Keywords: verification, repeatability, reproducibility, glucose, coefficient of variation.
Volume 38, Issue 1, November 2022, Pages 86–92
D. Benzekri1, A. Boujmad2, Asmaa Morjan3, and N. Kamal4
1 Laboratoire de biochimie, CHU Ibn Rochd de Casablanca, Morocco
2 Laboratoire de biochimie, CHU Ibn Rochd de Casablanca, Morocco
3 Laboratoire de biochimie, CHU Ibn Rochd, Casablanca, Morocco
4 Laboratoire de biochimie, Hôpital Ibn Rochd, CHU Ibn Rochd, Casablanca, Morocco
Original language: French
Copyright © 2022 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The verification/validation of a method is a necessary prerequisite before any activity in a medical biology laboratory. Also, it is important to remember the control of the risks associated with any dosing method as well as the impact of pre-analytical conditions on the post-analytical step. The objective of this work is to evaluate the analytical performance of serum and urinary glucose assay using the hexokinase enzymatic method of the manufacturer Abbott on the Alinity automaton of the same manufacturer. The verified parameters are reproducibility and repeatability, through the results of internal quality controls retrieved by creating procedures in the section of validation/verification of methods in our software: Big. The results of the performance evaluation in terms of reproducibility and repeatability demonstrate a perfect compliance of this method of serum and urine glucose determination by the Abbott kit on the Alinity PLC of the same manufacturer.
Author Keywords: verification, repeatability, reproducibility, glucose, coefficient of variation.
Abstract: (french)
La vérification et validation d’une méthode est un préalable nécessaire avant toute activité dans un laboratoire de biologie médicale. Aussi, il est important de rappeler la maitrise des risques liés à toute méthode de dosage ainsi que l’impact des conditions pré-analytiques sur l’étape post-analytique. Ce travail a pour objectif d’évaluer les performances analytiques du dosage du glucose sérique et urinaire par la méthode enzymatique à l’hexokinase du fabriquant Abbott sur automate Alinity du même fabriquant. Les paramètres vérifiés sont la reproductibilité et la répétabilité, au travers des résultats des contrôles internes de qualité récupérés en créant des procédures dans la rubrique dossier de validation/vérification de méthodes dans notre logiciel: Big. Les résultats de l’évaluation des performances en termes de reproductibilité et de répétabilité démontrent une conformité parfaite de cette méthode, enzymatique à l’hexokinase, du dosage du glucose sérique et urinaire par le kit Abbott sur l’automate Alinity du même fabriquant.
Author Keywords: vérification, répétabilité, reproductibilité, glucose, coefficient de variation.
How to Cite this Article
D. Benzekri, A. Boujmad, Asmaa Morjan, and N. Kamal, “Verification of the serum and urinary Glucose assay method by enzymatic hexokinase method on the Alinity automaton at the biochemistry laboratory-CHU Ibn Rochd in Casablanca, Morocco,” International Journal of Innovation and Applied Studies, vol. 38, no. 1, pp. 86–92, November 2022.
