The verification/validation of a method is a necessary prerequisite before any activity in a medical biology laboratory. Also, it is important to remember the control of the risks associated with any dosing method as well as the impact of pre-analytical conditions on the post-analytical step. The objective of this work is to evaluate the analytical performance of serum and urinary glucose assay using the hexokinase enzymatic method of the manufacturer Abbott on the Alinity automaton of the same manufacturer. The verified parameters are reproducibility and repeatability, through the results of internal quality controls retrieved by creating procedures in the section of validation/verification of methods in our software: Big. The results of the performance evaluation in terms of reproducibility and repeatability demonstrate a perfect compliance of this method of serum and urine glucose determination by the Abbott kit on the Alinity PLC of the same manufacturer.