[ Transmission du paludisme dans la commune de à Karimama au Nord-Est du Bénin ]
Volume 37, Issue 3, October 2022, Pages 569–576
Anges Yadouleton1, Gado Dramane2, BADOU Yvette3, TCHIBOZO Carine4, HOUNKANRIN Gildas5, Falilath SANOUSSI6, and Lamine Baba-Moussa7
1 Centre de Recherche Entomologique de Cotonou, Benin
2 University of Sciences, Arts and Techniques of Natitingou, Ecole Normale Supérieure de Natitingou, P.O. Box 72, Natitingou, Benin
3 Laboratoire des Fièvres Hémorragiques Virales et des Arbovirus, Benin
4 Laboratoire des Fièvres Hémorragiques Virales et des Arbovirus, Benin
5 Laboratoire des Fièvres Hémorragiques Virales et des Arbovirus, Benin
6 Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Benin
7 Department of Biochemistry, University of Abomey-Calavi, Faculty of Sciences and Techniques, Laboratory of Biology and Molecular Typing in Microbiology, 05 BP 1604 Cotonou, Benin
Original language: French
Copyright © 2022 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Background: In order to know the level of malaria transmission in the city of Karimama in the north East of Benin, an entomological study was carried out in this city from January to December 2020. Method : Firstly, the study was based on sampling adults mosquitoes by Human Landing Catches (HLC) in two villages performed monthly over two consecutive nights (8:00 PM to 6:00 AM) in 4 randomly selected compounds. These populations of mosquitoes were completed by Indoor Pyrethrum Spray Catches (PSC) in 10 other selected compounds; the same compounds in each sampling method being consistently used throughout the study. The head-thoraces of these females from the human landing catches were tested for the presence of CircumSporozoite Protein (CSP). Finally, Knocked down mosquitoes falling on white bed sheets were preserved for identification of species and characterization of molecular forms within the An. gambiae complex were performed using Polymerase Chain Reaction (PCR). Results: i)-During the year of study, 9.019 female mosquitoes were caught by PSC against 21.474 by HLC among which, 26% were Anopheles species from HLC; ii)- In this city surveyed, the main malaria parasite Plasmodium falciparum was transmitted by Anopheles gambiae s.s where transmission was high from June to October during the rainy season and declined during the dry season (December-May); iii) - The Entomological Inoculation Rates (EIRs) were significantly higher (138 infection bites/person/night) during the rainy season than those obtained during the dry season (138 infection bites/person/night) (P<0.05). Conclusion: The present study showed that malaria transmission is unimodal in the city of Karimama and the main malaria parasite, Plasmodium falciparum was transmitted by Anopheles gambiae s.s. These findings will be very useful for National Malaria Control Program authorities in the choice of the method to fight against malaria in this city.
Author Keywords: Malaria, Transmission, Anopheles gambiae, Karimama.
Volume 37, Issue 3, October 2022, Pages 569–576
Anges Yadouleton1, Gado Dramane2, BADOU Yvette3, TCHIBOZO Carine4, HOUNKANRIN Gildas5, Falilath SANOUSSI6, and Lamine Baba-Moussa7
1 Centre de Recherche Entomologique de Cotonou, Benin
2 University of Sciences, Arts and Techniques of Natitingou, Ecole Normale Supérieure de Natitingou, P.O. Box 72, Natitingou, Benin
3 Laboratoire des Fièvres Hémorragiques Virales et des Arbovirus, Benin
4 Laboratoire des Fièvres Hémorragiques Virales et des Arbovirus, Benin
5 Laboratoire des Fièvres Hémorragiques Virales et des Arbovirus, Benin
6 Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Benin
7 Department of Biochemistry, University of Abomey-Calavi, Faculty of Sciences and Techniques, Laboratory of Biology and Molecular Typing in Microbiology, 05 BP 1604 Cotonou, Benin
Original language: French
Copyright © 2022 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: In order to know the level of malaria transmission in the city of Karimama in the north East of Benin, an entomological study was carried out in this city from January to December 2020. Method : Firstly, the study was based on sampling adults mosquitoes by Human Landing Catches (HLC) in two villages performed monthly over two consecutive nights (8:00 PM to 6:00 AM) in 4 randomly selected compounds. These populations of mosquitoes were completed by Indoor Pyrethrum Spray Catches (PSC) in 10 other selected compounds; the same compounds in each sampling method being consistently used throughout the study. The head-thoraces of these females from the human landing catches were tested for the presence of CircumSporozoite Protein (CSP). Finally, Knocked down mosquitoes falling on white bed sheets were preserved for identification of species and characterization of molecular forms within the An. gambiae complex were performed using Polymerase Chain Reaction (PCR). Results: i)-During the year of study, 9.019 female mosquitoes were caught by PSC against 21.474 by HLC among which, 26% were Anopheles species from HLC; ii)- In this city surveyed, the main malaria parasite Plasmodium falciparum was transmitted by Anopheles gambiae s.s where transmission was high from June to October during the rainy season and declined during the dry season (December-May); iii) - The Entomological Inoculation Rates (EIRs) were significantly higher (138 infection bites/person/night) during the rainy season than those obtained during the dry season (138 infection bites/person/night) (P<0.05). Conclusion: The present study showed that malaria transmission is unimodal in the city of Karimama and the main malaria parasite, Plasmodium falciparum was transmitted by Anopheles gambiae s.s. These findings will be very useful for National Malaria Control Program authorities in the choice of the method to fight against malaria in this city.
Author Keywords: Malaria, Transmission, Anopheles gambiae, Karimama.
Abstract: (french)
Dans le but de connaître le niveau de transmission du paludisme dans la ville de Karimama, une étude entomologique a été réalisée dans cette ville de janvier à décembre 2020. Dans un premier temps, des captures de moustiques sur appât humain ont été effectuées dans deux villages à un rythme de 2 nuits consécutives par mois. Ces captures sur appât humain ont été complétées par des captures au pyrèthre effectuées à l’intérieur des maisons et ceci 2 fois par mois dans 10 habitations choisies également dans les mêmes zones de capture. Dans un deuxième temps, le thorax des moustiques capturés sur appât humain a été utilisé pour rechercher la positivité en protéine circum sporozoïte (CS) de Plasmodium falciparum par la méthode de titrage immunoenzymatique (ELISA-CSP). Enfin, la technique de Polymerase Chain Reaction (PCR) a été utilisée dans le but de connaître les différentes espèces du complexe Anopheles gambiae assurant la transmission du paludisme. Il ressort de ces travaux que : i) 21.474 moustiques adultes ont été capturés sur appât humain contre 9.019 par aspersion intradomiciliaire avec une population de Anopheles gambiae qui représentait 26% des captures sur appât humain; ii) La transmission du paludisme est assurée par Anopheles gambiae s.s avec une forte incidence pendant la saison pluvieuse (Juin - Novembre) et une déclinaison pendant la saison sèche (décembre-mai) ; iii) Le Taux d’Inoculation Entomologique en est significativement plus important en saison pluvieuse (138 piqures infectantes par personne par mois (pi/h/mois)) qu’en saison sèche (8pi/h/mois) (P<0,05). La présente étude a montré que la transmission du paludisme est unimodale dans la ville de Karimama et est assurée en grande partie par An. gambiae s.s. Ces résultats obtenus seront d’une grande utilité pour la prise de décision en matière du choix de lutte anti vectorielle contre le paludisme dans la ville de Malanville.
Author Keywords: Paludisme, transmission, Anopheles gambiae, Karimama.
How to Cite this Article
Anges Yadouleton, Gado Dramane, BADOU Yvette, TCHIBOZO Carine, HOUNKANRIN Gildas, Falilath SANOUSSI, and Lamine Baba-Moussa, “Malaria transmission in the district of Karimama in North-East of Benin,” International Journal of Innovation and Applied Studies, vol. 37, no. 3, pp. 569–576, October 2022.
