The present study was conducted to investigate the biological degradation of Zearalenone using by Iraqi probiotic 2% (w/v) and yeast filtrate of Saccharomyces boulardii using one of the mammalian system (white mouse ) which had an active influence in removing or reduction of toxic effects of mycotoxin Zearalenone on liver and kidney tissues of males white mice that extracted from fungal isolate Fusarium graminearum produced Zearalenone at concentration (2 mg / kg body weight) . As observed a clear improvement in liver and kidney tissues for group of mice that fed contaminated diet by Fusarium graminearum produced Zearalenone with Iraqi probiotic and yeast filtrate compared with positive control ( group of mice fed contaminated diet by Fusarium graminearum produced Zearalenone ) that caused the histological changes in liver and kidney tissues ,which represented by congestion vascular with necrosis and degeneration in hepatic cells in addition to reduction in glycoprotein granules. While, the histological changes in kidney tissue included congestion vascular and degeneration in distal and proximal tubules. The results of liver enzymes showed significant differences in positive group for three enzymes ALS , ALT and ALP reached ( 15.66, 14.66, 25.66 ) IU/ L respectively, compared with negative control (group of mice did not fed contaminated diet by Fusarium graminearum produced Zearalenone ) which reached for three enzymes ALS , ALT and ALP (15.66,22.00,13.33) IU/ L respectively. However, the results of the interaction between mice fed contaminated diet by Fusarium graminearum produced Zearalenone with Iraqi probiotic and yeast filtrate were shown significant increasing for ALS, ALT, ALP enzymes in addition to urea concentrations in kidney tissue compared with positive control.