A study of microalgae of the Bandama River was carried out from samples taken from February to October 2013 using 20 μm mesh wide plankton net. This study uses a light microscope and Scanning Electron Microscope (M.E.B.) which allows a better observation of the fine structure of the walls of the organisms. Seventy-five (75) taxa were identified, distributed among 27 genera, 13 families, 4 order and 2 classes. Among these, 12 taxa are mentioned for the first time in the algae flora of Côte d'Ivoire. This study contributes to increase our knowledge on Chlorophyta. Results of this study can be used for teaching and algae taxonomy studies.