Turraea heterophylla Smith (Meliaceae) is a species used in Ivorian’s traditional for its antimalarial and aphrodisiac properties. The objectives of this study are the phytochemical screening, the determination of polyphenols and flavonoids present in the leaves and the determination of the antibacterial activity of the methanolic extract of the leaves. The screening phytochemical was carried out using chemical characterization tests. The determination of total polyphenols and total flavonoids was carried out using a spectrophotomer. Antibacterial activity was assessed using the agar well diffusion method against Staphylococcus aureus (ATCC 25923), Escherichia coli (CIP 54127AF) and Pseudomonas aeruginosa (CIP 103467). Phytochemical screening revealed the presence of polyterpenes, steroids, alkaloids, saponins, flavonoids and phenolic compounds. The assay indicates a very high concentration of flavonoids and polyphenols in the ethyl acetate and aqueous extracts. In terms of biological tests, the study indicates that the methanolic extract of the leaves of T. heterophylla has bacteriostatic properties against the germs tested with MIC values greater than 3000 µg / mL.