In order to contribute to the knowledge of the culicidofauna of Loango national park, Gabon, an exploratory entomological survey was conducted in this area. Adult mosquitoes were captured in the vicinity of the Yatouga camp using CDC-light traps. These captures took place from February 20 to 27, 2020, from 5: 00 p.m. to 7: 00 a.m. and the captured mosquitoes were identified under binocular magnifying glass using morphological criteria. A total of 380 mosquitoes were collected. Morphological identifications of the captured specimens underlined the presence of 16 mosquito species especially Aedes circumluteolus (0.5%), Anopheles marshallii (15.5%), Anopheles obscurus (0.5%), Anopheles paludis (3.4%), Anopheles tenebrosus (0.5%), Coquilletidia aurites (0. 3%), Culex cinereus (1. 6%), Culex rubinotus (0. 5%), Culex decens (0. 5%), Ficalbia malfeyti (0. 5%), Mansonia africana (50. 5%), Uranotenia bilineata (1. 1%), Uranotenia cavernicola (13. 2%), Uranotenia caliginosa (0. 5%), Uranotenia mashonaensis (2. 1%), and Uranotenia nigromaculata (8. 7%). The genera Mansonia (50.5%), Uranotenia (25.6%) and Anopheles (20%) were the most abundant, while the genera Aedes, Coquilletidia, Ficalbia and Culex were very poorly represented with less than 3.9%. Most of the mosquitoes collected are known in other parts of Gabon and Africa for their vector role in the transmission of pathogens to humans and wildlife. There is an urgent need for a longitudinal study of the culicidofauna of this protected area, an habituation zone of ecotourism and great ape.