In tropical environments, parasitosis is a public health problem. This study aims to describe the evolution of the prevalence of intestinal parasitosis diagnosed at the laboratory of the University Hospital of Abomey-Calavi/So-Ava in the Atlantic Department of southern Benin in West Africa from 2010 to 2020. Each stool sample was examined directly with physiological water and stained with Lugol’s stain. From 2010 to 2020, 2348 patients benefited from a parasitological examination of stools at the laboratory of the University Hospital of Abomey-Calavi/So-Ava. 181 samples were positive (8%). 53% of the patients with parasitic disease were female. Children aged 0 to 5 years represent 51% of the parasitized patients. 97.90% of the parasites identified during the parasitological examination of stools belong to the group of protozoa. Entamoeba histolytica is the most observed parasite species (64.64%), followed by Entamoeba coli (27.76%), Trichomonas intestinalis (3.30%), Giardia lamblia (2.20%), Ascaris lumbricoides (1.6%) and Trichuris trichiura (0.5%). An effective control of intestinal parasitosis in Benin will require the eradication of intestinal protozoosis.