Through its involvement in a quality approach, the medical biology laboratory (MBL) seeks above all to satisfy the needs of its customers. The question is whether the production processes and improvements undertaken by the biologist are converging towards optimum performance. In order to do this, an effective evaluation process must be set up to assess the extent to which the MBL is meeting the requirements and objectives it has set itself. The aim of this paper is to identify the performance indicators of an MBL’S operational process and to develop a dashboard for evaluating its performance. For this study, we surveyed the literature to identify the various indicators used to measure business process performance. Once the indicators had been selected, we proceeded to contextualize and validate them by organizing a focus group. This focus group enabled us to add certain indicators and eliminate others, and to design a dashboard that offers a global and synthetic vision, and represents a turnkey solution for biologists to monitor laboratory activity in a quest for effectiveness and efficiency.