Anthracnose disease of mango contributes to a huge loss of mango fruits in côte d’Ivoire. This disease is the main pre- and post-harvest fungal disease infecting mango trees worldwide, and represents the 2nd major constraint to mango production and export in Côte d’Ivoire. However, information on the causal agent of this disease in Côte d’Ivoire remains scarse but présuméd to be Colletotrichum gloeosporioides as reported in early studies that were based on morphological characteristics. Since emerging information evidenced on one hand a possible intraspecific diversity within Colletotrichum gloeosporioides and on the other the existence of other emerging anthracnose causing agents, it was important to thoroughly identify these in North Côte d’Ivoire one of the main mango growing region. 41 fungal isolates were collected from diseased mango fruits in North Côte d’Ivoire, of which forty were morphologically identified as Colletotrichum gloeosporioides and one as Fusarium sp. Further molecular studies using ITS identified Colletotrichum gloeosporioides exhibiting an intraspecific diversity and Fusarium concentricum as the causal agents of anthracnose disease in mango in North Côte d’Ivoire.