This study was conducted in order to describe the determinants of public transport hygiene in Isiro town, in Haut-Uélé Province, DR Congo during the period from 8 February to 8 June 2023. The cross-sectional method supported by the survey questionnaire and direct observation of devices for maintaining hygiene in agencies and buses enabled us to collect the data. After analysis, the following results were recorded: - All the material devices relating to public transport hygiene are totally absent. - The majority of respondents reported vomiting on the bus (100%), not wearing a mask (57%), lack of knowledge about hygiene on public transport (62%), eating on public transport (100%) and smokers on the bus (83%). - The main consequences of hygiene problems are the presence of insects on the bus (83%), the presence of odours on the bus (70%) and poor hygiene on public transport (67%). In view of these results, it can be concluded that hygiene in public transport is still a major concern in the town of Isiro. As a result, training for agency managers and drivers and awareness-raising among passengers should be considered in order to avoid potential public health risks.