Autoimmune liver diseases are chronic liver diseases caused by the attack of the immune system against liver cells. They result from a variety of factors, including environmental, genetic and infectious. This study aims to compare the seropositivity of autoantibodies associated with autoimmune hepatopathy between patients previously exposed to HBV, HIV and these two viruses simultaneously, with patients who have never been in contact with HBV and HIV. We conducted a retrospective case-control study at Ibn Rochd University Hospital in Casablanca, from June 2023 to February 2024, on 80 patients divided into three groups: a control group without exposure to HBV and HIV, and two groups of cases involving HBV-infected patients and HIV. We evaluated the seroprevalence of autoantibodies associated with autoimmune hepatopathy using the immunodot technique with the LI7 DIV-24 kit and the Blue Diver automaton. Hbs antigen and total Hbc antibodies were detected by automated immuno-chemiluminescence. We found a seroprevalence of autoantibodies associated with autoimmune liver disease of 4% in controls, 30% in HBV-infected patients and 28.60% in HIV-infected patients. Anti-M2/nPDC and anti-Gp210 antibodies were positive in both HBV and HIV groups, anti-SLA predominated in HBV-infected patients, while anti-F-actin antibodies were more frequent in HIV-infected patients (P<0.02). In conclusion, our study, in agreement with several bibliographic data, suggests an established association between HBV, HIV and HBV-HIV co-infection and autoimmune hepatopathy.