Onychomycosis infection is considered as one of the main diseases that most of male and females are severing from this infections, The aim of this study was to survey the fungi contraction with clinical cases undergo Onychomycosis infections, and identification by phenotypic biochemical test, this study was performed from May2010 to May2011., clinical samples of Onychomycosis of at different age groups and sex were collected from Al-Dewania province. The results showed (88.48%) of patients undergo Onychomycosis and based on Occupations in house wives 41.81%, workers19.4, % farmers, 13.9%and followed by employments and students respectively, the age groups females were 51.32 % and males were 48.67%.Isolated 30 filamentous fungal species: Trichophyton rubrum, T. mentagrophytis, Geotrichum capitatum, Aspergillum fumigates, Asp. niger, Asp. oryzae, Asp. tamarri, and 14 isolates of Candida :C. albicans, C. tropicalis, C. kyfer, C. parapsilosis and C. krusei representative. Most fungi showed variable degrees in their susceptibilities to the antifungal agents. The CHROM agar medium and others tests were facililating the primary diagnosis for most Candida isolates.