This research aims to analyse the determinants of innovation and cooperation activities. Based on a sample of 364 Moroccan companies, we highlight the importance of the impact of cooperative relations allowing the company to access the knowledge and skills necessary for innovation that they couldn't acquire if they remained isolated. We particularly focus on the determinants of innovation of Moroccan companies, taking into account their cooperation leads to innovation in an innovation system. To respond to our question, we will develop the different theoretical approaches to innovation and a review of empirical literature on the innovative behavior of firms. Our results suggest that the probability of innovation is determined by internal and external firm attributes (size, industry, group membership and technological watch, etc.). On the other hand, it was found that the probability of innovation has increased more if the company launches simultaneously in research and development and cooperative relations for innovation.