The study of the Blue Cibacron (BC) treatment process through an statistical mathematical methods (SMM) allows us to determine a relation between nutritional variables, which are nitrogen source concentration (N), phosphorus source concentration (P) and vitamin mixture concentration (Vit.) on the decrease of coloration by bio-treatment. A complete factorial design (23) was made in order to determine the factors and their interactions which have a statistically significant influence on the studied response. Nitrogen source and vitamin mixture have a significant effect on the reduction of coloration; by contrast, Phosphorus source concentration does not have a significant effect. On the other hand, the interactions between the three factors have significant effects on the decrease of coloration. The discoloration activity is correlated with laccase activity.
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