Somatic embryogenesis is the process where cells acquire somatic embryogenic competence under specific conditions and interesting tool for clonal propagation. The auxinic herbicide picloram is used as a growth regulator in the induction of callus formation.The "tipa colorada" (Pterogyne nitens) is a legume that belongs to a monospecific genus and is seriously threatened, making it a priority to develop protocols that will produce regeneration of elite material in quantity and quality . This study aimed to evaluate the effect of picloram in the induction of somatic embryogenesis in "tipa colorada", for which the Murashige and Skoog medium was used at 100 % of its salt formulation, supplemented with five concentrations of Picloram (0.82; 1.65; 2.48; 3.31 and 4.14?M). At 30 days, color, callus consistency and percent callus formation was evaluated. Character embryogenic callus was determined by microscopic analysis It was possible to induce a 93.78 and 95.22 % of embryogenic callus creamy color, with picloram at concentrations of 3.31?M and 4.14?M respectively.