Study on the interlayer has developed associated with in-depth exploration and development of oil fields, which is an indispensable part to characterize reservoir heterogeneity. This study relates to a comprehensive research method of interlayer in rhythmic layering in continental low permeability reservoirs. During the process of waterflooding development, distribution characteristics of interlayer in the single well, plane, cross-well, and well group should be analyzed based on identification and genesis of interlayer. And thus the three-dimensional model of interlayer could be constructed in order to reveal its spatial distribution characteristics in continental reservoirs. Practice in continental oil fields has shown that the type and distribution of interlayer play an important role in controlling remaining oil. Mastering the distribution rule of interlayer can better explore the distribution of remaining oil. Through in-depth analysis on the interlayer, better development results could be achieved by taking different measures to trap the potential. Also, utilizations of interlayer results for the analysis of injection connectivity could facilitate petroleum engineers to adjust the injection layers, and thereby increasing the efficiency of oil field development.