The aim of this study was in one hand to characterize Mimosa pigra populations in three ponds in the Niokolo Koba National Park and in the other hand to determine an efficient controlling method of this invasive species. We characterized M. pigra populations by estimating at each pond total seed production per plant, number of infructescences per plant, number of pods per infructescence, number of seeds per pod, variability in seed weight, seed floating capacity, and germination rate. To achieve the second part of our goal, an experiment had been conducted during three years and consisted to compare three control methods such as