Polymorphisms of the glutathione S-transferases (GSTs), which are involved in the cellular oxidative and antioxidant mechanisms of the xenobiotic substances and carcinogens, represents a factor that increases the risk of developing cancer. We aimed to determine in a case-control study (82 patients and 152 controls) a possible association between the GSTT1, GSTM1 and GSTP1 gene polymorphisms and susceptibility to non-Hodgkin lymphoma (NHL) in a Romanian population. GTSs genotypes were obtained using the multiplex polymerase chain reaction (PCR) and the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Increased frequencies of the GSTM1 null genotype were observed in the patients (51.22%) with NHL and in controls (56.58%). No associations were observed between GSTP1 Ile/Val + Val/Val and GSTM1 null genotypes and risk of NHL, while an increased risk for GSTT1 null genotype was noticed without statistical significance. We did not find differences for the combined GST gene polymorphisms and risk of NHL between patients and controls. Also, no differences between patients’ demographic and clinical characteristics and GTSs genotypes were detected (p>0.05, for all comparisons). Therefore, our research suggests that GSTM1, GSTT1, and GSTP1 genotypes do not contribute to the risk of developing NHL.