Chlorophyll in the leaves is one of the main components of photosynthesis. We studied the fluorescence chloroplast papaya leaves using solvents such as acetone and ethanol (95o) of various concentrations at room temperature. Chlorophyll fluorescence is a useful technique in plant physiology. Fluorescence spectra of chloroplasts in solvents with various concentrations and under different excitation wavelengths (365 nm, 390 nm and blue) were investigated. Chlorophyll fluorescence is characterized by two peaks, one in the red, near 690 nm, and the other in the far red, near 740 nm. The fluorescence spectra measurements were taken using a spectrofluorimeter. Our results show that complex chlorophyll carriers are dissociated for high concentrations. Our results also show the evolution of peak positions with the solvent strength due to partial separations of complex chloroplasts.