Entrobiasis is a nematode infection caused by Enterobius vermicularis, there have been no reports on the infection rates of enterobiasis in Sidi-Bel-Abbes city. A cross-sectional study among schools in 10 districts was conducted in order to estimate the prevalence of this infection among preschool and school children less than 8 years of age, between April and June 2017. A total of 300 samples were examined microscopically for the presence of worm eggs collected by touching transparent tape to anal area. The overall prevalence of enterobiasis was 17,67%, and girls (26,5%) showed more infection rate compared to boys (16,92%), the prevalence of infection with age of 5 and 6 years old (23,4% and 25% respectively) was more than 7 years old children (13,43 %), prevalence of sucking thumb or fingers was 41,51%. Prevalence of enterobiasis has decreased by increasing the daily hand-washing frequency, cleaning hands one time (36,84%), two times (23,96%), three times (17,07%). When analyzing the association between the presence of enterobiasis and each variable separately, the only one showing statistically significant association was sucking thumb (p=0,019) and the symptoms of pruritus ani, abdominal pain, enuresis and insomnia (p=0,0001 respectively). Prevalence of E.vermicularis is relatively high and should not be underestimated.