Volume 5, Issue 3, March 2014, Pages 192–199
Edet Uwem Okon1, Gihan Hammed2, Poussy Abu El Wafa3, Opetiti Abraham4, Nicole Case5, and Elecia Henry6
1 Department of Biotechnology, School of Science at Medway, University of Greenwich, London, United Kingdom
2 Department of Biotechnology, School of Science at Medway, University of Greenwich, London, United Kingdom
3 Department of Biotechnology, School of Science at Medway, University of Greenwich, London, United Kingdom
4 Department of Biotechnology, School of Science at Medway, University of Greenwich, London, United Kingdom
5 Department of Biotechnology, School of Science at Medway, University of Greenwich, London, United Kingdom
6 Department of Biotechnology, School of Science at Medway, University of Greenwich, London, United Kingdom
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The success of gene therapy depends on the choice of a suitable vector that is biocompatible and efficient in delivering therapeutic DNA into disease cells. After more than two decades, such an ideal vector is still a wish. Viral vectors though naturally evolved to transfect cells are immunogenic. As alternatives, non-viral vectors such as polyethyleneimine have been exploited. We decided to investigate the in-vitro cytotoxicity of branched polyethyleneimine 800D, 25kD and linear 20kD on HeLa and Vero cells. At exponential phase, cells were exposed to polymers at concentration range of 0.5 to 1000mg/ml. Cells were MTT assayed after 24, 48 and 72hours for viability (IC50). Linear PEI was less toxic than the branched PEI in both cells. The IC50 (mg/ml) values (Mean
Author Keywords: HeLa, Vero, Polyethyleneimine, Poly L-lysine, Cytotoxicity, Dextran.
Edet Uwem Okon1, Gihan Hammed2, Poussy Abu El Wafa3, Opetiti Abraham4, Nicole Case5, and Elecia Henry6
1 Department of Biotechnology, School of Science at Medway, University of Greenwich, London, United Kingdom
2 Department of Biotechnology, School of Science at Medway, University of Greenwich, London, United Kingdom
3 Department of Biotechnology, School of Science at Medway, University of Greenwich, London, United Kingdom
4 Department of Biotechnology, School of Science at Medway, University of Greenwich, London, United Kingdom
5 Department of Biotechnology, School of Science at Medway, University of Greenwich, London, United Kingdom
6 Department of Biotechnology, School of Science at Medway, University of Greenwich, London, United Kingdom
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The success of gene therapy depends on the choice of a suitable vector that is biocompatible and efficient in delivering therapeutic DNA into disease cells. After more than two decades, such an ideal vector is still a wish. Viral vectors though naturally evolved to transfect cells are immunogenic. As alternatives, non-viral vectors such as polyethyleneimine have been exploited. We decided to investigate the in-vitro cytotoxicity of branched polyethyleneimine 800D, 25kD and linear 20kD on HeLa and Vero cells. At exponential phase, cells were exposed to polymers at concentration range of 0.5 to 1000mg/ml. Cells were MTT assayed after 24, 48 and 72hours for viability (IC50). Linear PEI was less toxic than the branched PEI in both cells. The IC50 (mg/ml) values (Mean
Author Keywords: HeLa, Vero, Polyethyleneimine, Poly L-lysine, Cytotoxicity, Dextran.
How to Cite this Article
Edet Uwem Okon, Gihan Hammed, Poussy Abu El Wafa, Opetiti Abraham, Nicole Case, and Elecia Henry, “In-vitro cytotoxicity of Polyethyleneimine on HeLa and Vero Cells,” International Journal of Innovation and Applied Studies, vol. 5, no. 3, pp. 192–199, March 2014.
