Volume 7, Issue 1, July 2014, Pages 329–342
Isabel A. Guillén1, Julio R. Fernández2, Daniel O. Palenzuela3, Santiago Dueñas4, Junsong han5, Zhiming Zhang6, Xiaona Zhang7, Yang Gao8, Jamilet Miranda9, Ricardo Bringas10, and Lidia I Novoa11
1 Center for Genetic Engineering and Biotechnology CIGB. Havana, Cuba. Ave 31 e/ 158 and 190, Havana, CP10600, Cuba
2 Center for Genetic Engineering and Biotechnology CIGB. Havana, Cuba. Ave 31 e/ 158 and 190, Havana, CP10600, Cuba
3 Center for Genetic Engineering and Biotechnology CIGB. Havana, Cuba. Ave 31 e/ 158 and 190, Havana, CP10600, Cuba
4 Center for Genetic Engineering and Biotechnology CIGB. Havana, Cuba. Ave 31 e/ 158 and 190, Havana, CP10600, Cuba
5 Shanghai Biochip Co. Ltd/National engineering center for biochip at Shanghai, No.151, Libing Rd., Zhangjiang Hi-Tech Park, Pudong 201203 Shanghai, China
6 Shanghai Biochip Co. Ltd/National engineering center for biochip at Shanghai, No.151, Libing Rd., Zhangjiang Hi-Tech Park, Pudong 201203 Shanghai, China
7 Shanghai Biochip Co. Ltd/National engineering center for biochip at Shanghai, No.151, Libing Rd., Zhangjiang Hi-Tech Park, Pudong 201203 Shanghai, China
8 Shanghai Biochip Co. Ltd/National engineering center for biochip at Shanghai, No.151, Libing Rd., Zhangjiang Hi-Tech Park, Pudong 201203 Shanghai, China
9 Center for Genetic Engineering and Biotechnology CIGB. Havana, Cuba. Ave 31 e/ 158 and 190, Havana, CP10600, Cuba
10 Center for Genetic Engineering and Biotechnology CIGB. Havana, Cuba. Ave 31 e/ 158 and 190, Havana, CP10600, Cuba
11 Center for Genetic Engineering and Biotechnology CIGB. Havana, Cuba. Ave 31 e/ 158 and 190, Havana, CP10600, Cuba
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Human peripheral blood is a useful material for biomedical research, since it can be used to investigate responses to treatment and predict diseases. However, various biological and technological factors produce a large degree of variation in blood of gene expression profiles. Our study was based on gene expression profiling analysis on peripheral blood of 45 healthy volunteers, 21 females and 24 men. The blood cells were concentrated, and the total RNA was isolated for the analysis of gene expression using the Affymetrix Gene Chip technology. The results were obtained by a fluorescent scanner, and the numerical data was analyzed using Bioconductor. Samples were clearly divided by gender through the unsupervised clustering analysis. 40 identified genes, differentiating samples by gender were analyzed according to their biological function and chromosomal location. Most of them are located on the X and Y chromosomes. These results provide new insights into the genetic makeup which distinguishes both sexes probably associated with diseases and sex determination.
Author Keywords: transcriptome, expression, gender, RNA, peripheral blood.
Isabel A. Guillén1, Julio R. Fernández2, Daniel O. Palenzuela3, Santiago Dueñas4, Junsong han5, Zhiming Zhang6, Xiaona Zhang7, Yang Gao8, Jamilet Miranda9, Ricardo Bringas10, and Lidia I Novoa11
1 Center for Genetic Engineering and Biotechnology CIGB. Havana, Cuba. Ave 31 e/ 158 and 190, Havana, CP10600, Cuba
2 Center for Genetic Engineering and Biotechnology CIGB. Havana, Cuba. Ave 31 e/ 158 and 190, Havana, CP10600, Cuba
3 Center for Genetic Engineering and Biotechnology CIGB. Havana, Cuba. Ave 31 e/ 158 and 190, Havana, CP10600, Cuba
4 Center for Genetic Engineering and Biotechnology CIGB. Havana, Cuba. Ave 31 e/ 158 and 190, Havana, CP10600, Cuba
5 Shanghai Biochip Co. Ltd/National engineering center for biochip at Shanghai, No.151, Libing Rd., Zhangjiang Hi-Tech Park, Pudong 201203 Shanghai, China
6 Shanghai Biochip Co. Ltd/National engineering center for biochip at Shanghai, No.151, Libing Rd., Zhangjiang Hi-Tech Park, Pudong 201203 Shanghai, China
7 Shanghai Biochip Co. Ltd/National engineering center for biochip at Shanghai, No.151, Libing Rd., Zhangjiang Hi-Tech Park, Pudong 201203 Shanghai, China
8 Shanghai Biochip Co. Ltd/National engineering center for biochip at Shanghai, No.151, Libing Rd., Zhangjiang Hi-Tech Park, Pudong 201203 Shanghai, China
9 Center for Genetic Engineering and Biotechnology CIGB. Havana, Cuba. Ave 31 e/ 158 and 190, Havana, CP10600, Cuba
10 Center for Genetic Engineering and Biotechnology CIGB. Havana, Cuba. Ave 31 e/ 158 and 190, Havana, CP10600, Cuba
11 Center for Genetic Engineering and Biotechnology CIGB. Havana, Cuba. Ave 31 e/ 158 and 190, Havana, CP10600, Cuba
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Human peripheral blood is a useful material for biomedical research, since it can be used to investigate responses to treatment and predict diseases. However, various biological and technological factors produce a large degree of variation in blood of gene expression profiles. Our study was based on gene expression profiling analysis on peripheral blood of 45 healthy volunteers, 21 females and 24 men. The blood cells were concentrated, and the total RNA was isolated for the analysis of gene expression using the Affymetrix Gene Chip technology. The results were obtained by a fluorescent scanner, and the numerical data was analyzed using Bioconductor. Samples were clearly divided by gender through the unsupervised clustering analysis. 40 identified genes, differentiating samples by gender were analyzed according to their biological function and chromosomal location. Most of them are located on the X and Y chromosomes. These results provide new insights into the genetic makeup which distinguishes both sexes probably associated with diseases and sex determination.
Author Keywords: transcriptome, expression, gender, RNA, peripheral blood.
How to Cite this Article
Isabel A. Guillén, Julio R. Fernández, Daniel O. Palenzuela, Santiago Dueñas, Junsong han, Zhiming Zhang, Xiaona Zhang, Yang Gao, Jamilet Miranda, Ricardo Bringas, and Lidia I Novoa, “Analysis of gene expression profile for gender in human blood samples,” International Journal of Innovation and Applied Studies, vol. 7, no. 1, pp. 329–342, July 2014.
