Volume 8, Issue 2, September 2014, Pages 529–538
AZIZ Faissal1, Juan Parrado Rubio2, Naaila Ouazzani3, Mohammed Dary4, Hamid Manyani5, Bruno Rodríguez Morgado6, and Laila Mandi7
1 National Center for Research and Studies on Water and Energy, University Cadi Ayyad, Marrakech, Morocco
2 Department of Biochemical and molecular biology, Faculty of Pharmacy, University of Seville, Spain
3 National Center for Research and Studies on Water and Energy, University Cadi Ayyad, Marrakech, Morocco
4 Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Spain
5 Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Spain
6 Department of Biochemical and molecular biology, Faculty of Pharmacy, University of Seville, Spain
7 National Center for Research and Studies on Water and Energy, University Cadi Ayyad, Marrakech, Morocco
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
In Morocco, storage reservoirs are particular systems of water supply in rural areas (a case study in Assif El Mal Valley). These reservoirs are fed by rainwater and/or directly from the river through opened channels, and are used without any treatment as a drinking water by the surrounding population.
This study was conducted to evaluate the bacterial contamination of drinking water reservoirs in this rural area using a molecular approach studying the 16S-rDNA bacterial diversity in water, via Polymerase Chain Reaction and Denaturing Gradient Gel Electrophoresis (PCR-DGGE) technique.
The application of PCR-DGGE techniques on stored water in the reservoirs showed a high bacterial diversity, including pathogens, namely Salmonella sp., E.coli, Sphingomonas spp. and Aeromonas sp., which indicated a high risk of infection for the user population.
Comparative cluster analyses of the DNA based fingerprints revealed the six studied reservoirs according a gradient accumulation of bacterial contaminants from upstream to downstream.
The molecular approach in this study gives a very helpful tool to confirm without any doubt the bacterial contamination of drinking water. Otherwise, this study provides an overview of the dominant bacterial groups in the traditional storage reservoirs of water in Moroccan rural area and the impact of environmental changes on bacterial diversity.
Author Keywords: bacterial diversity, water reservoir, 16S-rDNA, DGGE-PCR technique.
AZIZ Faissal1, Juan Parrado Rubio2, Naaila Ouazzani3, Mohammed Dary4, Hamid Manyani5, Bruno Rodríguez Morgado6, and Laila Mandi7
1 National Center for Research and Studies on Water and Energy, University Cadi Ayyad, Marrakech, Morocco
2 Department of Biochemical and molecular biology, Faculty of Pharmacy, University of Seville, Spain
3 National Center for Research and Studies on Water and Energy, University Cadi Ayyad, Marrakech, Morocco
4 Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Spain
5 Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Spain
6 Department of Biochemical and molecular biology, Faculty of Pharmacy, University of Seville, Spain
7 National Center for Research and Studies on Water and Energy, University Cadi Ayyad, Marrakech, Morocco
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
In Morocco, storage reservoirs are particular systems of water supply in rural areas (a case study in Assif El Mal Valley). These reservoirs are fed by rainwater and/or directly from the river through opened channels, and are used without any treatment as a drinking water by the surrounding population.
This study was conducted to evaluate the bacterial contamination of drinking water reservoirs in this rural area using a molecular approach studying the 16S-rDNA bacterial diversity in water, via Polymerase Chain Reaction and Denaturing Gradient Gel Electrophoresis (PCR-DGGE) technique.
The application of PCR-DGGE techniques on stored water in the reservoirs showed a high bacterial diversity, including pathogens, namely Salmonella sp., E.coli, Sphingomonas spp. and Aeromonas sp., which indicated a high risk of infection for the user population.
Comparative cluster analyses of the DNA based fingerprints revealed the six studied reservoirs according a gradient accumulation of bacterial contaminants from upstream to downstream.
The molecular approach in this study gives a very helpful tool to confirm without any doubt the bacterial contamination of drinking water. Otherwise, this study provides an overview of the dominant bacterial groups in the traditional storage reservoirs of water in Moroccan rural area and the impact of environmental changes on bacterial diversity.
Author Keywords: bacterial diversity, water reservoir, 16S-rDNA, DGGE-PCR technique.
How to Cite this Article
AZIZ Faissal, Juan Parrado Rubio, Naaila Ouazzani, Mohammed Dary, Hamid Manyani, Bruno Rodríguez Morgado, and Laila Mandi, “Evaluation of bacterial diversity in traditional drinking water reservoirs of rural areas: a molecular approach,” International Journal of Innovation and Applied Studies, vol. 8, no. 2, pp. 529–538, September 2014.
