Volume 13, Issue 4, December 2015, Pages 881–889
Smail AMALICH1, Nadia ZEKRI2, N'Dédianhoua K. SORO3, Kamal FADILI4, Youssef KHABBAL5, Malika MAHJOUBI6, EL Hassan AKDIM7, and Touryia ZAÏR8
1 Departement de Chimie, Equipe de recherche de chimie des molécules bioactives et de l'environnement, Faculté de sciences, Université Moulay Ismail, B.P. 11201, Zitoune, Meknès, Morocco
2 Laboratory of bioactive molecules Chemistry and environment, Moulay Ismail University of Sciences –Meknes, Morocco
3 Chemistry Department, Laboratory of Bioactive Molecules and Environment, Faculté de sciences, Université Moulay Ismail, Zitoune Meknès, 50000, Morocco
4 Departement de Chimie, Equipe de recherche de chimie des molécules bioactives et de l'environnement, Faculté de sciences, Université Moulay Ismail, B.P. 11201, Zitoune, Meknès, Morocco
5 Laboratory of pharmacology, Faculté de Médecine et pharmacie, Université Mohamed Ibn Abdallah Fès 300000, Morocco
6 Departement de Chimie, Equipe de recherche de chimie des molécules bioactives et de l'environnement, Faculté de sciences, Université Moulay Ismail, B.P. 11201, Zitoune, Meknès, Morocco
7 Laboratory of bioactive molecules Chemistry and environment, Moulay Ismail University of Sciences –Meknes, Morocco
8 Chemistry Department, Laboratory of Bioactive Molecules and Environment, Faculté de sciences, Université Moulay Ismail, Zitoune Meknès, 50000, Morocco
Original language: English
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Aromatic and medicinal plants are a great source of complex molecules exploited by mankind in many industrial fields. Currently, the increasing use of natural occurring compounds has been observed and this fact justifies the increasing production of certain medicinal and aromatic plants (MAP). In this work, we were interested to valorize Juniperus phoenicea, a native species from Moroccan Eastern High Atlas (Tounfite). This plant is used in traditional medicine for its medicinal properties to treat many infectious diseases. J. phoenicea's leaves and fruits harvested in the flowering period (October 2013) have been subjected to hydrodistillation using a Clevenger-type apparatus. The yields of extracted essential oils (EOs) are about 1.71% and 2.01% respectively for leaves and fruits. Analysis of the chemical composition of both EOs (from leaves and fruits), by gas chromatography coupled with mass spectrometry, has shown their richness in monoterpenes (81.05% and 51.73%) and sesquiterpenes (13.71% and 38.08%). Both EOs are widely dominated by ?-pinene (78.11% - 48.18% respectively). Antimicrobial activity of these oils was evaluated against four clinical strains: Escherichia coli, Pseudomonas aeruginosa; Staphylococcus aureus and Klebsiella pneumoniae. The results have shown that P. aeruginosa and S. aureus strains are sensitive to leaves' EO with inhibition areas that reached 23 mm and 26 mm respectively. The same minimum inhibitory concentration of 5.60
Author Keywords: Juniperus phoenicea, ?-pinene, Essential oils, antibacterial activity, bacterial strains.
Smail AMALICH1, Nadia ZEKRI2, N'Dédianhoua K. SORO3, Kamal FADILI4, Youssef KHABBAL5, Malika MAHJOUBI6, EL Hassan AKDIM7, and Touryia ZAÏR8
1 Departement de Chimie, Equipe de recherche de chimie des molécules bioactives et de l'environnement, Faculté de sciences, Université Moulay Ismail, B.P. 11201, Zitoune, Meknès, Morocco
2 Laboratory of bioactive molecules Chemistry and environment, Moulay Ismail University of Sciences –Meknes, Morocco
3 Chemistry Department, Laboratory of Bioactive Molecules and Environment, Faculté de sciences, Université Moulay Ismail, Zitoune Meknès, 50000, Morocco
4 Departement de Chimie, Equipe de recherche de chimie des molécules bioactives et de l'environnement, Faculté de sciences, Université Moulay Ismail, B.P. 11201, Zitoune, Meknès, Morocco
5 Laboratory of pharmacology, Faculté de Médecine et pharmacie, Université Mohamed Ibn Abdallah Fès 300000, Morocco
6 Departement de Chimie, Equipe de recherche de chimie des molécules bioactives et de l'environnement, Faculté de sciences, Université Moulay Ismail, B.P. 11201, Zitoune, Meknès, Morocco
7 Laboratory of bioactive molecules Chemistry and environment, Moulay Ismail University of Sciences –Meknes, Morocco
8 Chemistry Department, Laboratory of Bioactive Molecules and Environment, Faculté de sciences, Université Moulay Ismail, Zitoune Meknès, 50000, Morocco
Original language: English
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Aromatic and medicinal plants are a great source of complex molecules exploited by mankind in many industrial fields. Currently, the increasing use of natural occurring compounds has been observed and this fact justifies the increasing production of certain medicinal and aromatic plants (MAP). In this work, we were interested to valorize Juniperus phoenicea, a native species from Moroccan Eastern High Atlas (Tounfite). This plant is used in traditional medicine for its medicinal properties to treat many infectious diseases. J. phoenicea's leaves and fruits harvested in the flowering period (October 2013) have been subjected to hydrodistillation using a Clevenger-type apparatus. The yields of extracted essential oils (EOs) are about 1.71% and 2.01% respectively for leaves and fruits. Analysis of the chemical composition of both EOs (from leaves and fruits), by gas chromatography coupled with mass spectrometry, has shown their richness in monoterpenes (81.05% and 51.73%) and sesquiterpenes (13.71% and 38.08%). Both EOs are widely dominated by ?-pinene (78.11% - 48.18% respectively). Antimicrobial activity of these oils was evaluated against four clinical strains: Escherichia coli, Pseudomonas aeruginosa; Staphylococcus aureus and Klebsiella pneumoniae. The results have shown that P. aeruginosa and S. aureus strains are sensitive to leaves' EO with inhibition areas that reached 23 mm and 26 mm respectively. The same minimum inhibitory concentration of 5.60
Author Keywords: Juniperus phoenicea, ?-pinene, Essential oils, antibacterial activity, bacterial strains.
How to Cite this Article
Smail AMALICH, Nadia ZEKRI, N'Dédianhoua K. SORO, Kamal FADILI, Youssef KHABBAL, Malika MAHJOUBI, EL Hassan AKDIM, and Touryia ZAÏR, “Chemical Characterization and Antibacterial Evaluation of Juniperus phoenicea L. Leaves and Fruits' Essential Oils from Eastern High Atlas (Morocco),” International Journal of Innovation and Applied Studies, vol. 13, no. 4, pp. 881–889, December 2015.
