Volume 36, Issue 2, May 2022, Pages 493–501
Boutayna Khadiry1, Meriem El Kerch2, Brahim Farouki3, Brahim Takourt4, Jalila El Bakkouri5, and Hassan Fellah6
1 Laboratory of immunology and Serology, Ibn Rochd University Hospital, Casablanca, Morocco
2 Laboratory of immunology and Serology, Ibn Rochd University Hospital, Casablanca, Morocco
3 Laboratory of immunology and Serology, Ibn Rochd University Hospital, Casablanca, Morocco
4 Laboratoire d’Immuno-sérologie, CHU Ibn Rochd, Casablanca, Maroc
5 Laboratoire d’Immunologie, CHU Ibn Rochd, Faculté de Médecine et de Pharmacie, Université Hassan II, Casablanca, Morocco
6 Immunology Laboratory, Faculty of Medicine and Pharmacy, Casablanca, Morocco
Original language: English
Copyright © 2022 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Autoimmune diseases are marked by the presence of more or less specific autoantibodies for each of them. The detection and identification of these autoantibodies seem to be the pillars of the diagnosis. The aim of our study is to evaluate the ELISA technique compared to the immunodot, for the detection of extractable nuclear antigens antibodies Sm, SSA and SSB. This is a retrospective, comparative study of biological diagnostic techniques carried out over 6 months. 86 sera from patients tested by the immunodot for the detection of extractable nuclear antigens antibodies were analyzed by an ELISA test (AESKULISA) for the detection of anti-Sm, anti-SSA and anti-SSB antibodies. Sensitivities, specificities, PPVs, NPVs and the correlation index were calculated respectively for each of the three kits AESKULISA Sm (57.14%, 97.46%, 66.6%, 97.46%, 94%), AESKULISA SSA (53.84 %, 97.87%, 95.45%, 71.87%, 77.90%) and AESKULISA SSB (16.66%, 100%, 100%, 81.92%, 82.55%). Our study found low levels of sensitivity of the ELISA technique compared to the immunodot, which could be explained by the nature of the antigenic substrates, the coating procedures and the cut-off levels used by kits manufacturers. The immunodot appears to be more sensitive and more specific for the detection of anti-SSB antibodies and more sensitive for the detection of anti-SSA 52kDa antibodies. Indeed, a combination of two or more methods is to be recommended in order to optimize the relevance of the diagnostic test for the screening of anti-ENA antibodies.
Author Keywords: autoantibodies, autoantigens, antinuclear, horseradish peroxidase, fluorescence.
Boutayna Khadiry1, Meriem El Kerch2, Brahim Farouki3, Brahim Takourt4, Jalila El Bakkouri5, and Hassan Fellah6
1 Laboratory of immunology and Serology, Ibn Rochd University Hospital, Casablanca, Morocco
2 Laboratory of immunology and Serology, Ibn Rochd University Hospital, Casablanca, Morocco
3 Laboratory of immunology and Serology, Ibn Rochd University Hospital, Casablanca, Morocco
4 Laboratoire d’Immuno-sérologie, CHU Ibn Rochd, Casablanca, Maroc
5 Laboratoire d’Immunologie, CHU Ibn Rochd, Faculté de Médecine et de Pharmacie, Université Hassan II, Casablanca, Morocco
6 Immunology Laboratory, Faculty of Medicine and Pharmacy, Casablanca, Morocco
Original language: English
Copyright © 2022 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Autoimmune diseases are marked by the presence of more or less specific autoantibodies for each of them. The detection and identification of these autoantibodies seem to be the pillars of the diagnosis. The aim of our study is to evaluate the ELISA technique compared to the immunodot, for the detection of extractable nuclear antigens antibodies Sm, SSA and SSB. This is a retrospective, comparative study of biological diagnostic techniques carried out over 6 months. 86 sera from patients tested by the immunodot for the detection of extractable nuclear antigens antibodies were analyzed by an ELISA test (AESKULISA) for the detection of anti-Sm, anti-SSA and anti-SSB antibodies. Sensitivities, specificities, PPVs, NPVs and the correlation index were calculated respectively for each of the three kits AESKULISA Sm (57.14%, 97.46%, 66.6%, 97.46%, 94%), AESKULISA SSA (53.84 %, 97.87%, 95.45%, 71.87%, 77.90%) and AESKULISA SSB (16.66%, 100%, 100%, 81.92%, 82.55%). Our study found low levels of sensitivity of the ELISA technique compared to the immunodot, which could be explained by the nature of the antigenic substrates, the coating procedures and the cut-off levels used by kits manufacturers. The immunodot appears to be more sensitive and more specific for the detection of anti-SSB antibodies and more sensitive for the detection of anti-SSA 52kDa antibodies. Indeed, a combination of two or more methods is to be recommended in order to optimize the relevance of the diagnostic test for the screening of anti-ENA antibodies.
Author Keywords: autoantibodies, autoantigens, antinuclear, horseradish peroxidase, fluorescence.
How to Cite this Article
Boutayna Khadiry, Meriem El Kerch, Brahim Farouki, Brahim Takourt, Jalila El Bakkouri, and Hassan Fellah, “Evaluation of the ELISA technique compared to Immunodot in the screening of extractable nuclear antigens antibodies Sm, SSA and SSB,” International Journal of Innovation and Applied Studies, vol. 36, no. 2, pp. 493–501, May 2022.
