Vigna unguiculata (L.) Walp plant was efficiently regenerated using cotyledonary nodes as explant. The explants were excised from 3 days old seedling grown in vitro on MS medium containing 3 mg/l BAP. They were cultured on MS medium containing BAP (2,5 mg/l) combinated with different dose of NAA (0, 0,1 and 0,5 mg/l) for shoot induction. Regeneration frequency varied with growth regulator combinations in the medium. A maximum number of 9,6 shoots per explant was recorded on MS medium containing 2.5 mg/l BAP. Increase in the ANA concentration of the culture medium decreased the number of shoots. Regenerated shoots were elongated on MS medium containing 1,25 mg/l BAP. The elongated shoots were rooted on MS rooting medium without hormone. The rooted plants were transferred to soil with a survival rate of 80%.
Water stress is the major environmental stresses that affect agricultural production worldwide, especially in arid and semi-arid regions. This research investigated the effect of water deficit on five wheat cultivars grown in the greenhouse until anthesis. Mild (60%) and severe (30%) water deficit regimes were used with a control of 100% soil field capacity. The effect of water stress on parameters was investigated including; Relative water content (RWC), pigment content (chl a, chl b, carotenoids, chl tot), proline, soluble sugars, rate of K+ and Na+ were determined.
The results indicated that the effect of water deficit on biochemical parameters depended on the combination of water stress regime and wheat cultivars. The analyses carried show that under water deficit stress RWC was sharply reduced due a combination of leaf growth reduction and abscission. Water deficits impose leaf chlorophyll content decrease. In addition, wheat genotypes a more significant accumulation of solubles sugars than of proline. It results from them also a rate high from Na+ and a low rate of K+ at the time of the stress. Finally, the contribution of the physio-biochimicals parameters in the adaptation of the durum wheat depends closely on the genotypes and intensity of the hydrous deficit.
Saline soils are an unfavourable environment for the growth of most legumes. The aim of this work is to compare the biochemical behaviour of three species of bean (Vigna radiata L., Vigna mungo L., and Vigna angularis L.) under salt stress conditions. In order to assess this behaviour, we analysed polyphenols and flavonoids in the roots and leaves. The results reveal variability in the accumulation of polyphenols and flavonoids, depending on the organ of the plant, the species, and the intensity of the saline treatment.
The processes involved in the development of a performance culture are influenced not only by genetic factors but also by the intervention of environmental factors. Soil salinity is a permanent threat to the survival of plants. The choice of a salt tolerant species would be a solution to this constraint. This study aims to assess the impact of salt stress during the vegetative stage of okra (Abelmoschus esculentus L.).Plants were stressed with different concentrations of NaCl from 0, 50, 100, 150 to 200 mM NaCl for one week. The results obtained show that the application of stress results in a moderate decrease in the relative water content. Chlorophyll a, b, and total carot
The effect of salt stress on antioxidative activity was studied in two species of halophytes Atriplex: halimus and canescens. The plants were exposed to 100, 300 and 600 mM l-1 NaCl for 30 days. The parameter of oxidative stress induced by salt, malondialdehyde (MDA) and the activity of peroxidase (POD) are determined. The results show that in terms of lipid peroxidation, the two species are very little or not affected by the salt for all concentrations applied. This reflects a better protection of cell membranes against oxidative damaging effects of salt. However, in terms of peroxidase activity, variability of responses is observed. This variability is a function of the salt concentration, the organ and species.