Cotton fabrics were cationized with a cationic agent (Chromatech9414) then dyed with Brazilwood natural dye using ferric chloride as a mordant in one bath at three stages by exhaustion method. This process successes to impart cationic sites on cotton fabrics surface which improve its substantivity to natural dye beside save energy and water. The effect of (Chromatech9414) concentration on the nitrogen content was studied. Factors affected the dyeing stage such as dye conc., pH value, temperature and time of dyeing were investigated.The effect of cationic agent concentration and ferric chloride conc. on the colour strength of dyed cotton fabric with Brazilwood natural dye were observed. The best results were obtained at 8% Brazilwood natural dye, pH6 , 60 min., 60oC for dyeing stage at 6%(Chromatech9414) and 8g/l ferric chloride. The effect of mordant, dyeing pH and cationic agent concn. on the colour hue were also investigated .The wettability and the fastness properties of dyed samples were assessed.The changes in the fiber surface, by cationization ,were evaluated using scanning electron microscopy(SEM).