The aim of this study is to verify if the endemic cholera around Lake Kivu is consecutive to the colonization of plankton by V. cholerae. We undertook the search of V. cholerae germs in samples of water from this Lake then trying their elimination in removing zooplankton by filtration. Thus, from November 2011 to June 2013, we analyzed 64 samples of coastal water from 3 different sites of the Lake Kivu; one site in a rural basin (Ishungu) and 2 sites in a urban basin (Bukavu ). The detection and numeration of V. cholerae were made by culture methods completed by biochemical tests. Filtration was done thanks to zooplankton
The evolution over time of organoleptic and physico-chemical properties of hen eggs sold in Kisangani (DR Congo) markets was evaluated in terms of number of days after spawning. The results show that the majority of eggs sold (54.7%) are older than 30 days. The physicochemical parameters including volume, density, pH and water content decrease with time. The pH changed to slightly basic (7.5 before 8 days) to slightly acidic (6.8 after two months) while the water content is 77.4 to 69.3% for the same period of time. The organoleptic parameters are also changed with time. Notable differences can be noticed in the visual aspects of the yellow and white of the egg, odor and flavor. The duration time of transport, poor packaging and storage conditions are at the root of this deterioration in the quality of eggs.
The use of great apes Pharmacopoeia or zoo-pharmacognosy is a very promising strategy for the treatment of human diseases due to the phylogenetic proximity of humans and non-human primates (NHP) as Bonobos. In total, more than 40 direct fields' observations of bonobos have been performed for five months. A high number of direct observations were recorded in August followed by September while there was less observations in June. The average size and composition of the group for August is estimated at 19 individuals with 8 males, 9 females and 2 young whereas the size recorded in September was of 12 individuals including 5 males, 6 females and 1 young. Physicochemical and blood-biochemical analysis results performed on bonobos urine within their natural habitat shown the presence of bilirubin in some urine specimens (a formation of an azo derivative with a diazonium salt). The presence of a lot of leukocytes in urine is an indication of Plasmodium infection since we are in Malaria endemic regions. These results confirm the evidence of great ape pathological condition and then these could be considered as a biological model for testing and/or validating the self-medication hypothesis and the anti-hemolytic effects of plants consumed by NHP that could be profitable to the sickle cell individuals. The survey revealed that the pharmacopoeia of bonobos of Lomako Nature Reserve consists of 35 plant species. The bioassay test showed that Treculia africana, a plant being part of the diet of the bonobos, inhibits the sickling of red blood cells in vitro in hypoxic conditions and can serve as a source of bioactive metabolites for promoting an antisickling nutritherapy in Democratic Republic of the Congo. The relationship between the pharmacopoeia of apes and sickle cell anemia is an original approach. Thus, it opens up new perspectives for the treatment of this hemoglobinopathy through modulating effects of secondary metabolites of plant origin on the epi-genome.