The aim of this study is to verify if the endemic cholera around Lake Kivu is consecutive to the colonization of plankton by V. cholerae. We undertook the search of V. cholerae germs in samples of water from this Lake then trying their elimination in removing zooplankton by filtration. Thus, from November 2011 to June 2013, we analyzed 64 samples of coastal water from 3 different sites of the Lake Kivu; one site in a rural basin (Ishungu) and 2 sites in a urban basin (Bukavu ). The detection and numeration of V. cholerae were made by culture methods completed by biochemical tests. Filtration was done thanks to zooplankton