Introduction and purpose: The serum protein profile is a very important examination for the diagnosis, monitoring, surveillance and evaluation of certain inflammatory, immune and nutritional diseases, but its prescription remains a little limited. According to the recommendations of the good practices in biology, the prescription of this examination must be carried out under precise conditions. In our context, this technique is poorly prescribed by physicians. The objective of this work is to assess the state of the art of internal and residents doctors regarding the serum protein profile. Method: This is a descriptive, quantitative, exploratory and cross-sectional study supported by a 22 questions questionnaire, with closed questions in numbers of 4, 14 multiple-choice questions and 4 closed-ended questions from 59 physicians. This questionnaire evaluates their knowledge and their need for serum protein profile training as well as their expectation of benefits at the biochemistry laboratory. Results of the study: The exploitation of the results showed a lack of formation in protein profile. 96.3% of resident and internal pediatric physicians did not receive training in this area. 37.5% have unsatisfactory theoretical knowledge of the targeted protein profile. 40.7% of responders indicated that satisfaction with the biochemistry laboratory's performance is moderately satisfied concerning the response time. Conclusion: Training in protein profile is considered necessary for the prescribing doctors in order to consolidate their knowledge for a more useful prescription for the purpose of an improved diagnosis.