Volume 33, Issue 3, August 2021, Pages 522–535
Zoa Parfait Alexis1, Fotso2, Tene Tayo Martial3, and Djocgoue Pierre-François4
1 Department of Plant Biology, University of Yaounde I, Faculty of Science, P.O. Box 817, Yaounde, Cameroon
2 Department of Plant Biology, University of Bamenda, High Teacher’s Training College Bambili, P.O. Box 39, Bamenda, Cameroon
3 Laboratory of Phytoprotection and Plant Valorization, Biotechnology Center, University of Yaounde I, Messa, P.O. Box 3851, Yaounde, Cameroon
4 Department of Plant Biology, University of Yaounde I, Faculty of Science, P.O. Box 817, Yaounde, Cameroon
Original language: English
Copyright © 2021 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Okra is a very popular vegetable fruit due to its nutritional and therapeutic potential, but its valorization is still marginalized in agricultural research centers. However, it possesses secondary metabolites not yet identified in the scientific literature and involved in the antioxidant defense system. This work aimed at assessing the effect of soil amendment on biochemical parameters of Abelmoschus esculentus leaf and fruit extracts, involved in the defense against ROS. It appears that the bioformulation used, highly influence some growth parameters and physicochemical parameters of soil. The screening of the different secondary metabolites was done by GC/MS performed with an Agilent 7890A GC coupled to an Agilent MSD 5975C inert mass spectrometer. The free radical scavenging activity was assessed by DPPH (2, 2-diphenyl-l-picrylhydrazyl); ABTS (2,2'-azino-bis- (3-ethylbenzothiazoline6-sulfonic acid)) reduction tests. The reducing power towards ferric ion (FRAP) was also assessed. It was determined that the leaf extract from the plot treated with the Trichoderma harzianum and Bacillus amyloliquefaciens generated more secondary metabolites than the control extract. This treated extract also showed high IPs at peak concentrations on the three assays DPPH, ABTS and FRAP which are respectively 90.37±1.21%; 91.47±1.01; 85.40 ± 0.14% against 62.55±7.28%; 67.83±4.56; 36.43 ± 0.12% for the control with a highly significant difference (p-value<0.001). The okra fruit extract where GPx, CAT and SOD were assessed, showed a highly significant difference (p-value<0.0001) among these enzymes and according to treatments. Further analysis of our study shows that these beneficial microorganisms positively influenced the agromorphological parameters and play an important role in the improvement of the biochemical parameters involved in the antioxidant activity of Abelmoschus esculentus.
Author Keywords: Abelmoschus esculentus, Trichoderma harzianum and Bacillus amyloliquefaciens, antioxidant scavenging activity, secondary metabolites.
Zoa Parfait Alexis1, Fotso2, Tene Tayo Martial3, and Djocgoue Pierre-François4
1 Department of Plant Biology, University of Yaounde I, Faculty of Science, P.O. Box 817, Yaounde, Cameroon
2 Department of Plant Biology, University of Bamenda, High Teacher’s Training College Bambili, P.O. Box 39, Bamenda, Cameroon
3 Laboratory of Phytoprotection and Plant Valorization, Biotechnology Center, University of Yaounde I, Messa, P.O. Box 3851, Yaounde, Cameroon
4 Department of Plant Biology, University of Yaounde I, Faculty of Science, P.O. Box 817, Yaounde, Cameroon
Original language: English
Copyright © 2021 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Okra is a very popular vegetable fruit due to its nutritional and therapeutic potential, but its valorization is still marginalized in agricultural research centers. However, it possesses secondary metabolites not yet identified in the scientific literature and involved in the antioxidant defense system. This work aimed at assessing the effect of soil amendment on biochemical parameters of Abelmoschus esculentus leaf and fruit extracts, involved in the defense against ROS. It appears that the bioformulation used, highly influence some growth parameters and physicochemical parameters of soil. The screening of the different secondary metabolites was done by GC/MS performed with an Agilent 7890A GC coupled to an Agilent MSD 5975C inert mass spectrometer. The free radical scavenging activity was assessed by DPPH (2, 2-diphenyl-l-picrylhydrazyl); ABTS (2,2'-azino-bis- (3-ethylbenzothiazoline6-sulfonic acid)) reduction tests. The reducing power towards ferric ion (FRAP) was also assessed. It was determined that the leaf extract from the plot treated with the Trichoderma harzianum and Bacillus amyloliquefaciens generated more secondary metabolites than the control extract. This treated extract also showed high IPs at peak concentrations on the three assays DPPH, ABTS and FRAP which are respectively 90.37±1.21%; 91.47±1.01; 85.40 ± 0.14% against 62.55±7.28%; 67.83±4.56; 36.43 ± 0.12% for the control with a highly significant difference (p-value<0.001). The okra fruit extract where GPx, CAT and SOD were assessed, showed a highly significant difference (p-value<0.0001) among these enzymes and according to treatments. Further analysis of our study shows that these beneficial microorganisms positively influenced the agromorphological parameters and play an important role in the improvement of the biochemical parameters involved in the antioxidant activity of Abelmoschus esculentus.
Author Keywords: Abelmoschus esculentus, Trichoderma harzianum and Bacillus amyloliquefaciens, antioxidant scavenging activity, secondary metabolites.
How to Cite this Article
Zoa Parfait Alexis, Fotso, Tene Tayo Martial, and Djocgoue Pierre-François, “Effect of soil amendment with Trichoderma harzianum and Bacillus amyloliquefaciens bioformulation on biochemical parameters and antioxidant activity in Abelmoschus esculentus,” International Journal of Innovation and Applied Studies, vol. 33, no. 3, pp. 522–535, August 2021.
