Okra is a very popular vegetable fruit due to its nutritional and therapeutic potential, but its valorization is still marginalized in agricultural research centers. However, it possesses secondary metabolites not yet identified in the scientific literature and involved in the antioxidant defense system. This work aimed at assessing the effect of soil amendment on biochemical parameters of Abelmoschus esculentus leaf and fruit extracts, involved in the defense against ROS. It appears that the bioformulation used, highly influence some growth parameters and physicochemical parameters of soil. The screening of the different secondary metabolites was done by GC/MS performed with an Agilent 7890A GC coupled to an Agilent MSD 5975C inert mass spectrometer. The free radical scavenging activity was assessed by DPPH (2, 2-diphenyl-l-picrylhydrazyl); ABTS (2,2'-azino-bis- (3-ethylbenzothiazoline6-sulfonic acid)) reduction tests. The reducing power towards ferric ion (FRAP) was also assessed. It was determined that the leaf extract from the plot treated with the Trichoderma harzianum and Bacillus amyloliquefaciens generated more secondary metabolites than the control extract. This treated extract also showed high IPs at peak concentrations on the three assays DPPH, ABTS and FRAP which are respectively 90.37±1.21%; 91.47±1.01; 85.40 ± 0.14% against 62.55±7.28%; 67.83±4.56; 36.43 ± 0.12% for the control with a highly significant difference (p-value<0.001). The okra fruit extract where GPx, CAT and SOD were assessed, showed a highly significant difference (p-value<0.0001) among these enzymes and according to treatments. Further analysis of our study shows that these beneficial microorganisms positively influenced the agromorphological parameters and play an important role in the improvement of the biochemical parameters involved in the antioxidant activity of Abelmoschus esculentus.