This study aimed to evaluate the efficacy of the biopesticide Prolaly on the improvement of productivity and quality of tomato (Solanum lycopersicum L.). The study was carried out in a randomized complete block design with three (03) repetitions. Treatments were carried out once a fortnight, using a sprayer, 21 days after transplanting (DAT). Vegetative growth parameters, statistically identical for all treatments at 32 DAT, were statistically different at 75 DAT. Phenological stages did not vary between treatments. The entomological data obtained revealed that the TB biological treatment was more effective in controlling whiteflies but less effective against Helicoverpa armigera. Furthermore, two diseases were observed in the experimental plot. They are a virus related to the spoon leaf curl virus (TYLCV) and a fungal disease related to collar rot. The incidences of virus disease on tomato plants were 80, 80 and 100% for TC, TB and T0 treatments respectively. Regarding fungal disease, the incidences were 20, 30 and 50% for TC, TB and T0 treatments, respectively. However, the highest healthy fruit rate (56.34%) was obtained with the TB treatment with a net yield (6.15 t/ha) statistically identical (p = 0.0230262) to that of the TC treatment (6.31 t/ha). Plant extract formulations would be an alternative in the biological management of diseases and insect pests of vegetable crops.