This research in this aspect is necessary, because of PFASs are one kind of hydrophobic and hydrophilic chemicals, and the study of PFASs with protein used as a dissolved organic matter in this study is valuable to evaluate the ecological risk of this kind of chemicals. Fluorescence has been proven as a sensible method to provide qualitative and quantitative information on the PFAS-serum albumin interactions. This work will provide some information for appropriately understanding the interaction between PFASs and protein and illustrate its binding mechanisms at different concentration of protein, cations and pH. The results obtained from fluorescence spectra indicated that PFASs could quench the intrinsic fluorescence of protein through a static quenching procedure, with the effective quenching constants (K’sv) varying from 0.44 105 L mol-1 to 5.73 105 L mol-1. It infers that the complex of PFAS-protein was formed. In addition, the ionic strength and pH affected the effective quenching constant of PFASs bound to bovine albumin. Furthermore, with increase of pH from 6.5 to 8.5, reduction in the binding affinity of PFAS to bovine albumin and soy peptone were also observed. The interaction between perfluoroalkyl substances and protein using fluorescence spectroscopy, and the occurrence of electrostatic interactions with hydrophobic force in the binding also studied.

Numerous studies have shown the variability of the physico-chemical and microbiological characteristics of meteorite waters collected and stored in recovery tanks. This variability can have negative impacts for users, physico-chemical and microbiological analyzes reflecting the quality of rainwater collected downstream from the roof and stored in a concrete tank were carried out. The chosen site is a rainwater tank whose use is not limited to laundry, some households use it for washing, for domestic uses in the kitchen, and even for drinking. The objective of this work is to assess the risks to which users are exposed when consuming collected and stored rainwater. The hydrogen potential analysis revealed an average content of 7.05. The turbidity value is less than 5NTU.The conductivity has an average value of 0,01005 µs/cm. The quantity of chloride ion is very varied for each sample analyzed, with an average of 227.47 mg/l in Cl-. The samples show considerable number of total coliforms.