Introduction On 8 February 2013, a cluster of influenza AH1N1 in a fishing boat docked at the port of Dakhla was reported to the Regional Health Directorate of Oued Ed-Dahab Lagouira. An epidemiological investigation was conducted to identify the source of infection, control the outbreak and prevent its extension. Methods: The investigation of this case grouped AH1N1 began on 12 February. It involved 26 fishermen aboard a boat docked at the port of Dakhla. Data were collected using an individual questionnaire. Results: The occurrence of cases occurred between 1 and 10 February. The age of cases ranged from 26 to 55 years with an average of 40 (+ / - 8 years). Depending on the date of onset of symptoms, the first case dates back to February 1, 11 days before the start of the investigation. Among the 14 cases, 2 were confirmed for the AH1N1 virus. The last case dating back to February 10, and the epidemic peak was reached on February 4. Cases were all presented with fever, cough and ache. The overall attack rate was 14/26 (53.8%). Attack rate vary by age and disease and was significantly higher in the age group of 36-45 years. Conclusion: This investigation allowed us to document the AH1N1 outbreak in a fishing boat and the importance of early implementation of control measures at the onset of the first case in a community.