The effects of the diet fat type on some biochemical, growth and semen characteristics were studied in 32 males Muscovy ducks distributed into 4 comparable experimental groups of 8 birds. During a period of 12 weeks, each of the group was randomly fed one of the rations including the control diet (T) or one diet containing either soya bean oil (S), pig fat (G) or palm oil (P). Life weight of group G was significantly (P<0.05) lower as compared to other groups. The carcass yield and percentage of liver relative to life weight was similar (P>0.05) among groups. Diet containing Pig fat induced the lowest (P<0.05) heart percentage while Soya bean oil induced the highest. The highest (P<0.05) testis proportion (0.59%) was registered in control group and the lowest (0.16%) with Palm oil which also induced the highest (P<0.05) gizzard proportion. Pig fat induced the significantly (P<0.05) higher intra testicular protein as compared to the others treatments. Control group recorded the significantly (P<0.05) lower intra hepatic protein rate as compared to the pig fat group. The intra testicular cholesterol rate remained comparable (P>0.05) among treatment groups. For the hepatic cholesterol rate, value obtained with palm oil was significantly (P<0.05) higher than that of the control group. Sperm volume induced by pig fat was significantly (P<0.05) smaller than that of other treatments groups. Semen characteristics (concentration, motility, viability and pH) were not statistically (P>0.05) different among groups. The type of dietary fat except pig fat may therefore improve growth and reproduction performance of Muscovy duck.