In order to determine the morphometric characterization and molecular identification of cattle in Bangladesh, three districts, i.e., Pabna, Bogra and Jhenidah were selected. In each district, 15 outstanding different crossbred and local dairy female cattle's (cows) blood samples (total 45) and raw data were collected to carry out the study of molecular and morphometric characters respectively. These characters were observed to identify the genetic resources of cattle in the selected regions such as eye color, coat color, horn pattern, age, breed types, conception rate, litter size, milk production, lactation length, heat period and gestation period. The quantity of DNA were found to 198.30