Stored seeds of Phaseolus vulgaris L. in are the target of several insects like Acanthoscelides obtectus Say. These pests can cause losses greater than 80 % after six to seven months of storage. The present study aimed to evaluate the insecticidal effects of Moringa oleifera seeds on adults of A. obtectus in storage through two compositions (ie powdery and oily). Four concentrations of oils (3.33; 6.66; 9.99 and 13.33 μl/g), four powder concentrations (4; 16.67; 33.33 and 50 %) and one negative control (C0 = 0 μL / mL) for each composition were used. The experiment was conducted in the laboratory, with four repetitions. The results of the oil contact toxicity test revealed 100 % mortality at doses of 300 and 400 μL after two days of exposure. As for Moringa powder, the insecticidal effect was less pronounced for all doses with a mortality rate of 55 % at day 5 for doses 10 and 15 g. The inhibition of eggs laid and their viability is a function of the concentration of Moringa oil. On the other hand, the powder had no effect on the number of eggs laid and rather stimulated the viability of the eggs. Moringa oil prevents weight loss of stored bean seeds with a null loss percentage at doses of 300 and 400 μL. As for the powder, it had no effect on the weight loss of bean seeds in storage. The powder and oil of Moringa seeds did not affect the germination capacity of the seeds for all the doses tested. Moringa seed oil can therefore be exploited in the integrated control of the pest of common bean seeds in storage.

In Cameroon, mycoparasitic strains of T. asperellum used in biocontrol of cocoa black pod disease have shown inconsistencies in their effectiveness. One possibility to optimize their performances is within the frames of the integrated management programme involving a combination of chemical and biological control methods. However, implementation of such an approach strongly relies on the compatibility between the biocontrol agents and the conventional synthetic fungicides. This study aimed to assess in vitro the compatibility between two antagonistic strains of T. asperellum (PR11 and PR12) and six fungicides (Ridomil, Penncozeb, Beauchamp, Nordox, Golden Blue and Kocide) approved and regularly used to control P. megakarya. These strains were cultured on PDA media supplemented with five different concentration levels (0, 0.01, 0.1, 10 and 100 % of the recommended field dose) of the above-cited fungicides. Effects on conidial germination, vegetative growth and conidial production were measured to evaluate the compatibility between the tested fungicides and the T. asperellum strains. Results showed an almost complete inhibition of conidial germination for the highest concentrations for all the fungicides. Vegetative growth and conidiogenesis were also significantly affected by the recommended field dose for all tested fungicides. The physiological parameter index used to classify compatibility showed that all tested fungicides are incompatible with both strains of T. asperellum at recommended field doses. According to the results reported in this study, the synthetic fungicides used to reduce the incidence of cocoa black pod disease have negative effects on both antagonistic T. asperellum strains PR11 and PR12. This suggests that their use in conjunction with T. asperellum as part of a disease control scheme would only be possible at sub-optimal concentrations.