Volume 5, Issue 4, April 2014, Pages 333–343
Imane Wahby1, Iman Bennis2, Chakib Tilsaghani3, and Luis María Lubián4
1 Green Biotechnology Department. MASCIR (Morrocan Foundation for Advanced Sciences, Innovation and Research), Design Center, Avenue Mohamed El Jazouli, Madinat al Irfane, Rabat, Morocco
2 Green Biotechnology Department. MASCIR (Morrocan Foundation for Advanced Sciences, Innovation and Research), Design Center, Avenue Mohamed El Jazouli, Madinat al Irfane, Rabat, Morocco
3 Green Biotechnology Department. MASCIR (Morrocan Foundation for Advanced Sciences, Innovation and Research), Design Center, Avenue Mohamed El Jazouli, Madinat al Irfane, Rabat, Morocco
4 Instituto de Ciencias Marinas de Andalucía (ICMAN) Campus Universitario Río San Pedro, 11510 Puerto Real (Cádiz), Spain
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The decrease of petroleum reserves and the global increase in energy demand has resulted in the focus of research toward exploration of alternate fuels using biological renewable sources. Biodiesel is one of these renewable energy forms. Microalgae are considered one of the most promising sources for biodiesel production. However, the potential use of microalgae in this field still needs to be explored since only about twenty species has been studied from the approximately 30.000 known species. Thereby, screening work on new potentially lipid overproducer strains is actually an active field. An overview of potential applications of multi-parameter flow cytometry in development of a biodiesel production strategy using microalgae is detailed in this review. Rapid and simultaneous measurements of different physiological parameters indicating diversity in marine and freshwater microalgal communities, biomass quality such as biochemical composition and viability of individual cells, isolation of targeted cells and obtention of axenic cultures are applications of flow cytometry that enable monitoring and optimisation of production of lipids from microalgae.
Author Keywords: microalgae, biodiesel, flow cytometry, fluorescence, screening.
Imane Wahby1, Iman Bennis2, Chakib Tilsaghani3, and Luis María Lubián4
1 Green Biotechnology Department. MASCIR (Morrocan Foundation for Advanced Sciences, Innovation and Research), Design Center, Avenue Mohamed El Jazouli, Madinat al Irfane, Rabat, Morocco
2 Green Biotechnology Department. MASCIR (Morrocan Foundation for Advanced Sciences, Innovation and Research), Design Center, Avenue Mohamed El Jazouli, Madinat al Irfane, Rabat, Morocco
3 Green Biotechnology Department. MASCIR (Morrocan Foundation for Advanced Sciences, Innovation and Research), Design Center, Avenue Mohamed El Jazouli, Madinat al Irfane, Rabat, Morocco
4 Instituto de Ciencias Marinas de Andalucía (ICMAN) Campus Universitario Río San Pedro, 11510 Puerto Real (Cádiz), Spain
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The decrease of petroleum reserves and the global increase in energy demand has resulted in the focus of research toward exploration of alternate fuels using biological renewable sources. Biodiesel is one of these renewable energy forms. Microalgae are considered one of the most promising sources for biodiesel production. However, the potential use of microalgae in this field still needs to be explored since only about twenty species has been studied from the approximately 30.000 known species. Thereby, screening work on new potentially lipid overproducer strains is actually an active field. An overview of potential applications of multi-parameter flow cytometry in development of a biodiesel production strategy using microalgae is detailed in this review. Rapid and simultaneous measurements of different physiological parameters indicating diversity in marine and freshwater microalgal communities, biomass quality such as biochemical composition and viability of individual cells, isolation of targeted cells and obtention of axenic cultures are applications of flow cytometry that enable monitoring and optimisation of production of lipids from microalgae.
Author Keywords: microalgae, biodiesel, flow cytometry, fluorescence, screening.
How to Cite this Article
Imane Wahby, Iman Bennis, Chakib Tilsaghani, and Luis María Lubián, “Potential use of flow cytometry in microalgae-based biodiesel project development,” International Journal of Innovation and Applied Studies, vol. 5, no. 4, pp. 333–343, April 2014.
