Volume 7, Issue 3, August 2014, Pages 1148–1155
Reham A. El-Shafei1 and Abdelfattah H. Eladl2
1 Department of Pharmacology, Faculty of Veterinary Medicine, Mansoura University, Egypt
2 Department of Poultry Diseases, Faculty of Veterinary Medicine, Mansoura University, Egypt
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Background: Florfenicol (Ff) is a synthetic antibiotic with a broad antibacterial spectrum and high therapeutic effectiveness that was specifically developed for veterinary use as well as, doxcycline is an antibiotic synthetically derived from oxytetracycline.
Methods: In the present study, the in-vitro efficacy of Ff and doxcycline against Salmonella entertidis and Escherichia coli (Serotype O78) pathogens was determined using disc diffusion technique and Minimum inhibitory concentrations (MICs). In the other hand serum and tissue residual levels of Ff and doxcycline after intramuscular (i.m.) administration of 30 mg/kg and 250 mg/kg orally respectively to 5 weeks old chicken were determined using microbiological assay method with Bacillus subtilis ATCC 6633 as a reference organism.
Results: The results showed that both microorganisms were highly susceptible to Ff with lower MIC value than those of doxcycline. The peak concentration of florfenicol in serum was 5.34
Author Keywords: Florfenicol, Doxcycline, Bacillus subtilis, Drug residues.
Reham A. El-Shafei1 and Abdelfattah H. Eladl2
1 Department of Pharmacology, Faculty of Veterinary Medicine, Mansoura University, Egypt
2 Department of Poultry Diseases, Faculty of Veterinary Medicine, Mansoura University, Egypt
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Florfenicol (Ff) is a synthetic antibiotic with a broad antibacterial spectrum and high therapeutic effectiveness that was specifically developed for veterinary use as well as, doxcycline is an antibiotic synthetically derived from oxytetracycline.
Methods: In the present study, the in-vitro efficacy of Ff and doxcycline against Salmonella entertidis and Escherichia coli (Serotype O78) pathogens was determined using disc diffusion technique and Minimum inhibitory concentrations (MICs). In the other hand serum and tissue residual levels of Ff and doxcycline after intramuscular (i.m.) administration of 30 mg/kg and 250 mg/kg orally respectively to 5 weeks old chicken were determined using microbiological assay method with Bacillus subtilis ATCC 6633 as a reference organism.
Results: The results showed that both microorganisms were highly susceptible to Ff with lower MIC value than those of doxcycline. The peak concentration of florfenicol in serum was 5.34
Author Keywords: Florfenicol, Doxcycline, Bacillus subtilis, Drug residues.
How to Cite this Article
Reham A. El-Shafei and Abdelfattah H. Eladl, “Determination of Florfenicol and Doxcycline Residues in Chickens by Microbiological Assay,” International Journal of Innovation and Applied Studies, vol. 7, no. 3, pp. 1148–1155, August 2014.
