Volume 10, Issue 2, February 2015, Pages 459–465
Md. Touhiduzzaman khan1, Md. Obayedul Hoque Reza2, Md. Anisuzzaman Khan3, Md. Shahidul Haque4, Md. Shahidul Islam5, and Md. Badiuzzaman Khan6
1 Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
2 Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
3 Department of Environmental Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
4 Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
5 Department of Environmental Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
6 Department of Environmental Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
Original language: English
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Random Amplified Polymorphic DNA (RAPD) assay was conducted for molecular genetic analysis of six cowpea (Vigna unguiculata L. Walp germplasms) i.e. BARI Cowpea, BARI Felon-1, Ranirhat, Ruma, BF06001 and BF060011, to observed genetic variability and relatedness among them. Out of 15 decamer random primers, three were used to amplify genomic DNA and the primers yielded a total of 20 RAPD markers of which 11 were considered as polymorphic. The maximum number of bands (9) (25%) produced by the primer S1184, whereas the least number of bands (5) (15%) was generated by the primer OPA10. The average scorable and polymorphic bands produced by the three primers were 6.67, and 3.67, respectively. The percentage of polymorphic loci was 55. The UPGMA dendrogram based on Nei's (1972) genetic distance between different pairs were correlated with their sources of origin. The dendrogram indicated segregation of six germplasms of cowpea into two main clusters; the main clusters are further divided into sub clusters. In sub cluster I, BARI Cowpwa-1, Ranirhat and Ruma formed sub sub-cluster I; BARI Felon-1 formed alone sub sub-cluster II. Again, sub sub-cluster I; Ruma formed alone cluster and BARI Cowpea-1 and Ranirhat formed sub sub-cluster III.
Author Keywords: Genetic diversity, Vigna unguiculata, polymorphism, RAPD, marker.
Md. Touhiduzzaman khan1, Md. Obayedul Hoque Reza2, Md. Anisuzzaman Khan3, Md. Shahidul Haque4, Md. Shahidul Islam5, and Md. Badiuzzaman Khan6
1 Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
2 Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
3 Department of Environmental Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
4 Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
5 Department of Environmental Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
6 Department of Environmental Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
Original language: English
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Random Amplified Polymorphic DNA (RAPD) assay was conducted for molecular genetic analysis of six cowpea (Vigna unguiculata L. Walp germplasms) i.e. BARI Cowpea, BARI Felon-1, Ranirhat, Ruma, BF06001 and BF060011, to observed genetic variability and relatedness among them. Out of 15 decamer random primers, three were used to amplify genomic DNA and the primers yielded a total of 20 RAPD markers of which 11 were considered as polymorphic. The maximum number of bands (9) (25%) produced by the primer S1184, whereas the least number of bands (5) (15%) was generated by the primer OPA10. The average scorable and polymorphic bands produced by the three primers were 6.67, and 3.67, respectively. The percentage of polymorphic loci was 55. The UPGMA dendrogram based on Nei's (1972) genetic distance between different pairs were correlated with their sources of origin. The dendrogram indicated segregation of six germplasms of cowpea into two main clusters; the main clusters are further divided into sub clusters. In sub cluster I, BARI Cowpwa-1, Ranirhat and Ruma formed sub sub-cluster I; BARI Felon-1 formed alone sub sub-cluster II. Again, sub sub-cluster I; Ruma formed alone cluster and BARI Cowpea-1 and Ranirhat formed sub sub-cluster III.
Author Keywords: Genetic diversity, Vigna unguiculata, polymorphism, RAPD, marker.
How to Cite this Article
Md. Touhiduzzaman khan, Md. Obayedul Hoque Reza, Md. Anisuzzaman Khan, Md. Shahidul Haque, Md. Shahidul Islam, and Md. Badiuzzaman Khan, “Genetic Diversity Analysis of Cowpea by RAPD Markers,” International Journal of Innovation and Applied Studies, vol. 10, no. 2, pp. 459–465, February 2015.
