[ Efecto del picloram en la inducción de embriogénesis somática en Pterogyne nitens Tul. "tipa colorada" ]
Volume 11, Issue 3, June 2015, Pages 771–777
Maritza Vacca Molina1, Zulma Avilés2, María Luisa Cristina Bonomo3, and Lucía Díaz4
1 Cátedra de Fisiología Vegetal, Universidad Nacional de Salta, Facultad de Ciencias Naturales, Salta, Argentina
2 Cátedra de Fisiología Vegetal, Universidad Nacional de Salta, Facultad de Ciencias Naturales, Salta, Argentina
3 Cátedra de Introducción a la Biología, Universidad Nacional de Salta, Facultad de Ciencias Naturales, Salta, Argentina
4 Cátedra de Caña de Azúcar, Universidad Nacional de Tucumán, Facultad de Agronomía y Zootecnia, San Miguel de Tucumán, Argentina
Original language: Spanish
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Somatic embryogenesis is the process where cells acquire somatic embryogenic competence under specific conditions and interesting tool for clonal propagation. The auxinic herbicide picloram is used as a growth regulator in the induction of callus formation.The "tipa colorada" (Pterogyne nitens) is a legume that belongs to a monospecific genus and is seriously threatened, making it a priority to develop protocols that will produce regeneration of elite material in quantity and quality . This study aimed to evaluate the effect of picloram in the induction of somatic embryogenesis in "tipa colorada", for which the Murashige and Skoog medium was used at 100 % of its salt formulation, supplemented with five concentrations of Picloram (0.82; 1.65; 2.48; 3.31 and 4.14?M). At 30 days, color, callus consistency and percent callus formation was evaluated. Character embryogenic callus was determined by microscopic analysis It was possible to induce a 93.78 and 95.22 % of embryogenic callus creamy color, with picloram at concentrations of 3.31?M and 4.14?M respectively.
Author Keywords: Pterogyne, picloram, embryogenesis, forestry, legume.
Volume 11, Issue 3, June 2015, Pages 771–777
Maritza Vacca Molina1, Zulma Avilés2, María Luisa Cristina Bonomo3, and Lucía Díaz4
1 Cátedra de Fisiología Vegetal, Universidad Nacional de Salta, Facultad de Ciencias Naturales, Salta, Argentina
2 Cátedra de Fisiología Vegetal, Universidad Nacional de Salta, Facultad de Ciencias Naturales, Salta, Argentina
3 Cátedra de Introducción a la Biología, Universidad Nacional de Salta, Facultad de Ciencias Naturales, Salta, Argentina
4 Cátedra de Caña de Azúcar, Universidad Nacional de Tucumán, Facultad de Agronomía y Zootecnia, San Miguel de Tucumán, Argentina
Original language: Spanish
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Somatic embryogenesis is the process where cells acquire somatic embryogenic competence under specific conditions and interesting tool for clonal propagation. The auxinic herbicide picloram is used as a growth regulator in the induction of callus formation.The "tipa colorada" (Pterogyne nitens) is a legume that belongs to a monospecific genus and is seriously threatened, making it a priority to develop protocols that will produce regeneration of elite material in quantity and quality . This study aimed to evaluate the effect of picloram in the induction of somatic embryogenesis in "tipa colorada", for which the Murashige and Skoog medium was used at 100 % of its salt formulation, supplemented with five concentrations of Picloram (0.82; 1.65; 2.48; 3.31 and 4.14?M). At 30 days, color, callus consistency and percent callus formation was evaluated. Character embryogenic callus was determined by microscopic analysis It was possible to induce a 93.78 and 95.22 % of embryogenic callus creamy color, with picloram at concentrations of 3.31?M and 4.14?M respectively.
Author Keywords: Pterogyne, picloram, embryogenesis, forestry, legume.
Abstract: (spanish)
La embriogénesis somática es el proceso donde las células somáticas adquieren competencia embriogénica bajo condiciones específicas y constituye una herramienta de interés para la propagación clonal. El picloram es un herbicida auxínico utilizado como un regulador de crecimiento en la inducción de callogénesis. La "tipa colorada" (Pterogyne nitens) es una leguminosa que pertenece a un género monoespecífico y se encuentra seriamente amenazada; por lo que es prioritario desarrollar protocolos de regeneración que permitan la obtención de material élite en cantidad y en calidad. El presente trabajo tuvo como finalidad evaluar el efecto del picloram en la inducción de embriogénesis somática en "tipa colorada", para lo cual se utilizó el medio de cultivo de Murashige y Skoog al 100% de su formulación salina, suplementado con cinco concentraciones de Picloram (0.82; 1.65; 2.48; 3.31 y 4.14µM). A los 30 días, se evaluó coloración, consistencia del callo y el porcentaje de formación de callos. El carácter embriogénico de los callos fue determinado mediante análisis microscópico. Se logro inducir un 93,78 y 95,22 % de callos embriogénicos de coloración cremosa, con picloram en concentraciones de 3.31 µM y 4.14µM respectivamente.
Author Keywords: Pterogyne, picloram, embriogenesis, forestal, leguminosa.
How to Cite this Article
Maritza Vacca Molina, Zulma Avilés, María Luisa Cristina Bonomo, and Lucía Díaz, “Picloram effect on the induction of somatic embryogenesis in Pterogyne nitens Tul. "tipa colorada",” International Journal of Innovation and Applied Studies, vol. 11, no. 3, pp. 771–777, June 2015.
